OLIGONUCLEOTIDES-TRANSFERRING PREPARATIONS
Preparations for transferring efficiently oligonucleotides necessary in antisense therapy or the like into animal cells so as to be useful in treatment for various diseases, which comprises a collagen as an essential component are provided.
1 - 11 . (canceled)
12 . A method of inhibiting expression of a target mRNA in a subject comprising:
administering to the subject an article comprising an oligonucleotide of 5 to 30 nucleotides in length and a water soluble atelocollagen that has a degree of cross-linking of 3 or less.
13 . The method of claim 12 , in which the atelocollagen has a molecular weight of 300,000 to 900,000 daltons.
14 . The method of claim 12 , in which the atelocollagen is a water soluble type I atelocollagen.
15 . The method of claim 12 , in which the oligonucleotide has at least one phosphorothioate bond.
16 . The method of claim 12 , in which the oligonucleotide has a base sequence complementary to a sequence of a target mRNA or of a target mRNA precursor.
17 . The method of claim 12 , in which the oligonucleotide is a DNA or a DNA derivative.
18 . The method of claim 12 , in which the oligonucleotide is a RNA or a RNA derivative.
19 . A method of inhibiting expression of a target mRNA in a cell comprising:
contacting the cell with an article comprising an oligonucleotide of 5 to 30 nucleotides in length and a water soluble atelocollagen that has a degree of cross-linking of 3 or less.
20 . The method of claim 19 , in which the atelocollagen has a molecular weight of 300,000 to 900,000 daltons.
21 . The method of claim 19 , in which the atelocollagen is a water soluble type I atelocollagen.
22 . The method of claim 19 , in which the oligonucleotide has at least one phosphorothioate bond.
23 . The method of claim 19 , in which the oligonucleotide has a base sequence complementary to a sequence of a target mRNA or of a target mRNA precursor.
24 . The method of claim 19 , in which the oligonucleotide is a DNA or a DNA derivative.
25 . The method of claim 19 , in which the oligonucleotide is a RNA or a RNA derivative.