Gene and protein expression profiles associated with the therapeutic efficacy of irinotecan
View Patent ↗The present invention includes gene and protein expression profiles indicative of whether a cancer patient is likely to respond to treatment with irinotecan. By identifying such responsiveness, a treatment provider may determine in advance those patients who would benefit from such treatment, as well as identify alternative therapies for non-responders. The present invention further provide methods of using the gene and/or protein expression profiles and assays for identifying the presence of a gene and/or protein expression profile in a patient sample.
1. An assay for determining if a patient diagnosed with colon cancer is a responder to treatment with irinotecan comprising
(a) a means for determining expression levels in a tumor cell or tumor tissue, a sample which has been mounted in a tissue microarray (TMA), from the patient of each of ERBB2 consisting of the amino acid sequence of SEQ ID NO. 23, GRB7 consisting of the amino acid sequence of SEQ ID NO. 24, JNK1 kinase consisting of the amino acid sequence of SEQ ID NO. 26, BCL2 consisting of the amino acid sequence of SEQ ID NO. 27, MK167 consisting of the amino acid sequence of SEQ ID NO. 33, phospho-Akt consisting of the amino acid sequence of SEQ ID NO. 35, CD68 consisting of the amino acid sequence of SEQ ID NO. 36, and BAG 1 consisting of the amino acid sequence of SEQ ID NO. 37; wherein the means for determining the expression levels consist of one antibody for each protein; and
(b) a system for predicting at least one of response to irinotecan therapy (RR), late recurrence (LRec) or disease-related death (DRD), comprising a database storing information of differential expression of the proteins of (a) in a tumor cell or tumor tissue from the patient as compared to normal colon tissue or cells, along with clinical information of the patient from whom the sample is obtained, an algorithm to correlate the differential expression of proteins to the patient information, and scoring criteria based on total percentage of staining by each antibody.
2. The assay of claim 1 further comprising means for detecting the expression level of at least one reference protein in the cells or tissue of the patient diagnosed with colon cancer.
3. The assay of claim 2 wherein the reference protein is GAPD having the amino acid sequence of SEQ ID NO. 40.
4. The assay of claim 1 further comprising means for determining expression levels in a tumor cell or tumor tissue from the patient of Erk1 kinase consisting of the amino acid sequence of SEQ ID NO. 25, phospho-GSK-3-beta consisting of the amino acid sequence of SEQ ID NO. 28, MMP11 consisting of the amino acid sequence of SEQ ID NO. 29, CTSL2 consisting of the amino acid sequence of SEQ ID NO. 30, CCNBI consisting of the amino acid sequence of SEQ ID NO. 31, BIRC5 consisting of the amino acid sequence of SEQ ID NO. 32, STK6 consisting of the amino acid sequence of SEQ ID NO. 34, MRP14 consisting of the amino acid sequence of SEQ ID NO. 44 or GSTMI consisting of the amino acid sequence of SEQ ID NO. 38.
5. The assay of claim 1 , wherein the patient is a responder to treatment with irinotecan if the level of each of ERBB2 consisting of the amino acid sequence of SEQ ID NO. 23, GRB7 consisting of the amino acid sequence of SEQ ID NO. 24, JNK1 kinase consisting of the amino acid sequence of SEQ ID NO. 26, BCL2 consisting of the amino acid sequence of SEQ ID NO. 27, MK167 consisting of the amino acid sequence of SEQ ID NO. 33, phospho-Akt consisting of the amino acid sequence of SEQ ID NO. 35, CD68 consisting of the amino acid sequence of SEQ ID NO. 36 and BAG1 consisting of the amino acid sequence of SEQ ID NO. 37, is elevated in the tissue or cells as compared to the corresponding levels in normal colon tissue or cells.
6. The assay of claim 4 , wherein the patient is a responder to treatment with irinotecan if the level of Erk1 kinase consisting of the amino acid sequence of SEQ ID NO. 25, phospho-GSK-3-beta consisting of the amino acid sequence of SEQ ID NO. 28, MMP11 consisting of the amino acid sequence of SEQ ID NO. 29, CTSL2 consisting of the amino acid sequence of SEQ ID NO. 30, CCNB1 consisting of the amino acid sequence of SEQ ID NO. 31, BIRC5 consisting of the amino acid sequence of SEQ ID NO. 32, STK6 consisting of the amino acid sequence of SEQ ID NO. 34, MRP14 consisting of the amino acid sequence of SEQ ID NO. 44, or GSTM1 consisting of the amino acid sequence of SEQ ID NO. 38, is lower in the tissue or cells as compared to the corresponding levels in normal colon tissue or cells.
7. The assay of claim 4 , wherein the patient is a responder to treatment with irinotecan if the level of each of ERBB2 consisting of the amino acid sequence of SEQ ID NO. 23, GRB7 consisting of the amino acid sequence of SEQ ID NO. 24, JNK1 kinase consisting of the amino acid sequence of SEQ ID NO. 26, BCL2 consisting of the amino acid sequence of SEQ ID NO. 27, MK167 consisting of the amino acid sequence of SEQ ID NO. 33, phospho-Akt consisting of the amino acid sequence of SEQ ID NO. 35, CD68 consisting of the amino acid sequence of SEQ ID NO. 36 and BAG1 consisting of the amino acid sequence of SEQ ID NO. 37, is elevated in the tissue or cells as compared to the corresponding levels in normal colon tissue or cells, and if the level of Erk1 kinase consisting of the amino acid sequence of SEQ ID NO. 25, phospho-GSK-3-beta consisting of the amino acid sequence of SEQ ID NO. 28, MMP11 consisting of the amino acid sequence of SEQ ID NO. 29, CTSL2 consisting of the amino acid sequence of SEQ ID NO. 30, CCNB1 consisting of the amino acid sequence of SEQ ID NO. 31, BIRC5 consisting of the amino acid sequence of SEQ ID NO. 32, STK6 consisting of the amino acid sequence of SEQ ID NO. 34, MRP14 consisting of the amino acid sequence of SEQ ID NO. 44, or GSTM1 consisting of the amino acid sequence of SEQ ID NO. 38, is lower in the tissue or cells as compared to the corresponding levels in normal colon tissue or cells.
8. An assay for determining if a patient diagnosed with colon cancer is a responder to treatment with irinotecan consisting of
(a) a means for determining expression levels in a tumor cell or tumor tissue from the patient of each of Erk1 kinase consisting of the amino acid sequence of SEQ ID NO. 25, phospho-GSK-3-beta consisting of the amino acid sequence of SEQ ID NO. 28, MMP11 consisting of the amino acid sequence of SEQ ID NO. 29, CTSL2 consisting of the amino acid sequence of SEQ ID NO. 30, CCNBI consisting of the amino acid sequence of SEQ ID NO. 31, BIRC5 consisting of the amino acid sequence of SEQ ID NO. 32, STK6 consisting of the amino acid sequence of SEQ ID NO. 34, MRP14 consisting of the amino acid sequence of SEQ ID NO. 44 and GSTMI consisting of the amino acid sequence of SEQ ID NO. 38, wherein the means for determining the expression levels consist of one antibody for each protein; and
(b) a system for predicting at least one of response to irinotecan therapy (RR), late recurrence (LRec) or disease-related death (DRD), comprising a database storing information of differential expression of the proteins of (a) in a tumor cell or tumor tissue from the patient as compared to normal colon tissue or cells, along with clinical information of the patient from whom the sample is obtained, an algorithm to correlate the differential expression of proteins to the patient information, and scoring criteria based on total percentage of staining by each antibody.
9. The assay of claim 8 , wherein the patient is a responder to treatment with irinotecan if the level of each of Erk1 kinase consisting of the amino acid sequence of SEQ ID NO. 25, phospho-GSK-3-beta consisting of the amino acid sequence of SEQ ID NO. 28, MMP11 consisting of the amino acid sequence of SEQ ID NO. 29, CTSL2 consisting of the amino acid sequence of SEQ ID NO. 30, CCNB1 consisting of the amino acid sequence of SEQ ID NO. 31, BIRC5 consisting of the amino acid sequence of SEQ ID NO. 32, STK6 consisting of the amino acid sequence of SEQ ID NO. 34, MRP14 consisting of the amino acid sequence of SEQ ID NO. 44, and GSTM1 consisting of the amino acid sequence of SEQ ID NO. 38, is lower in the tissue or cells as compared to the corresponding levels in normal colon tissue or cells.
10. The assay of claim 1 wherein each antibody has a detectable label.
11. The assay of claim 1 further comprising a secondary antibody.
12. The assay of claim 11 wherein the secondary antibody is a biotinylated secondary antibody.
13. The assay of claim 12 further comprising peroxidase labeled streptavidin.