Water soluble cholesterol derivatives
View Patent ↗Four novel water soluble cholesterol derivative compounds are disclosed. These compounds have various applications in studies of membrane proteins, including drug screening and studies of receptor stability and folding. In one aspect the water soluble cholesterol derivatives disclosed may be used to replace cholesterol in micelle-solubilized membrane protein preparations.
1. Cholesterol-β-tetrasaccharide according to Formula I:
2. Cholesterol-α-tetrasaccharide according to Formula II:
3. Cholesterol-β-sulfated maltoside according to Formula III, wherein X is selected from the group consisting of Me 3 NH, NH 4 , and Na:
4. Diosgenin-β-tetrasaccharide according to Formula IV:
5. A composition comprising the cholesterol-β-tetrasaccharide according to claim 1 .
6. A composition comprising the cholesterol-α-tetrasaccharide according to claim 2 .
7. A composition comprising the cholesterol-β-sulfated maltoside according to claim 3 .
8. A composition comprising the diosgenin-β-tetrasaccharide according to claim 4 .
9. A method of reconstituting membrane proteins in a lipid bilayer, comprising:
providing membrane protein;
providing at least one cholesterol derivative selected from the group consisting of:
mixing the membrane protein and the at least one cholesterol derivative to reconstitute membrane protein suitable for in vitro biochemical and structural studies.
10. The method according to claim 9 , wherein the membrane protein is a G-protein coupled receptor (GPCR).
11. The method according to claim 9 , wherein the membrane protein is a transmembrane protein.