IP Library › Granted Patent US 8,120,760
Granted Patent B2
US 8,120,760 · App. 12/494,476 · Granted Feb 21, 2012

Method and apparatus for separating a composite liquid into at least two components and for determining the yield of at least one component

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Quick Facts
Patent No.
US 8,120,760
App. No.
12/494,476
Granted
Feb 21, 2012
Kind
B2
Abstract

Method and Apparatus for predicting the yield of a selected cellular component from a composite blood product by sensing the movement of the separated cellular component or another component during expression from a separation container to a collection container to produce a signal indicative of the movement, and predicting the yield of the separated cellular component from the signal.

Claims (51)

1. A method of predicting the yield of a selected cellular component

from a composite blood product comprising

centrifuging the composite blood product in a separation container to separate the composite blood product into at least the one selected cellular component and another component;

expressing one of the separated cellular component or the other component from the separation container to a collection container;

sensing the movement of the expressed component or the other component during the expressing step from the separation container to the collection container to produce a signal indicative of the expressed component; and

predicting the yield of the separated cellular component from the signal.

2. The method of claim 1 wherein the expressing step comprises expressing the separated cellular component.

3. The method of claim 2 wherein the sensing step comprises sensing the movement of the separated cellular component with a photocell.

4. The method of claim 3 wherein the sensing step further comprises the photocell sensing the interface between the separated selected cellular component and other cellular components in the composite blood product.

5. The method of claim 2 wherein the separated cellular component comprises buffy coat.

6. The method of claim 5 wherein the composite blood product is whole blood.

7. The method of claim 2 wherein the separated cellular component is a platelet component.

8. The method of claim 7 wherein the centrifuging step further comprises separating a plasma component from the composite blood product; and

expressing the plasma component from the separation container to a plasma collection container prior to expressing the platelet component.

9. The method of claim 7 wherein the composite blood product is whole blood.

10. The method of claim 7 further comprising

determining if the predicted yield is desirable for pooling of the separated platelet component; and

if the predicted yield is not desirable, halting the expressing of the separated platelet component or changing the expressing in favor of the collection of another separated component.

11. The method of claim 2 wherein the predicting step further comprises calculating the area under a portion of the signal representative of the movement of the separated cellular component.

12. The method of claim 11 wherein the calculating step further comprises calculating the definite integral under a portion of the signal curve of the signal.

13. The method of claim 2 wherein the sensing the movement step further comprises determining the volume of the expressed component from the leading edge and trailing edge of the signal.

14. The method of claim 13 wherein the predicting step further comprises predicting the yield from the volume expressed.

15. The method of claim 1 wherein the expressing step comprises expressing the other component.

16. The method of claim 2 wherein the expressing step further comprises squeezing the separation container to express the separated cellular component.

17. The method of claim 16 wherein the squeezing step comprises flowing hydraulic fluid to squeeze the separation container.

18. The method of claim 1 wherein the sensing the movement further comprises determining the volume of the expressed component from the leading edge and trailing edge of the signal.

19. The method of claim 18 wherein the predicting step further comprises predicting the yield from the volume expressed.

20. The method of claim 1 further comprising predicting the yield of a plurality of selected cellular components from a plurality of composite blood products on a centrifuge.

21. Apparatus for predicting the yield of a selected cellular component from a composite blood product comprising

a separation container;

at least one collection container;

a centrifuge for centrifuging the composite blood product in the separation container to separate the composite blood product into a selected cellular component and at least one other component;

a system for expressing one of the separated cellular component or the at least one other component from the separation container to the at least one collection container;

a sensor for sensing the movement of the expressed component during expression from the separation container to the at least one collection container to produce a signal indicative of the expressed component movement; and

a controller for predicting the yield of the expressed component from the signal.

22. The apparatus of claim 21 wherein the system for expressing comprises an hydraulic system for squeezing the separation container to express the expressed component.

23. The apparatus of claim 21 wherein the sensor comprises a photocell.

24. The apparatus of claim 21 wherein the expressed component is the separated cellular component.

25. The apparatus of claim 24 wherein the separated cellular component comprises buffy coat.

26. The apparatus of claim 24 wherein the separated cellular component comprises a platelet component.

27. The apparatus of claim 26 wherein the controller calculates the definite integral under a portion of the signal curve of the signal.

28. The apparatus of claim 26 wherein the composite blood product is whole blood.

29. The apparatus of claim 26 wherein the controller determines if the predicted yield is desirable for pooling of the separated platelet components and if the predicted yield is not desirable, changing the expressing of the separated platelet component in favor of the collection of another separated component.

30. The apparatus of claim 24 wherein the sensor senses the leading edge and the trailing edge of the separated cellular component layer; and

wherein the controller determines the volume of the expressed separated cellular component from the leading and trailing edges.

31. The apparatus of claim 30 wherein the controller predicts the yield of the separated cellular component from the volume.

32. The apparatus of claim 21 wherein the controller calculates the area under a portion of the signal representative of the movement of the expressed component.

33. The apparatus of claim 21 where the composite blood product is whole blood.

34. The apparatus of claim 21 wherein the sensor senses the movement of the other component to determine its volume;

the controller subtracts the volume of the other component from the overall volume of the composite blood product to determine the volume of the selected cellular component; and

the controller predicts the yield of the separated cellular component from its volume.