Bead Array Reader Based-Hemagglutination and Hemagglutination Inhibition Assay
Hemagglutination assays and hemagglutination inhibition assays were introduced in medical and virology practice more than 60 years ago. Since then, these assays have become important tools for measuring concentrations and strengths of viral cultures, the efficacy of the anti-viral immunization, and for studying the neutralizing capacity of virus-specific antibodies. The present invention comprises an improved hemagglutination inhibition assay (HAI), with at least about a 10-fold increase in sensitivity versus the traditional the HAI, to provide more accurate measurements of components in, for example, fluids from the in vitro MIMIC® system when assessing the effects of anti-viral vaccines (e.g., for seasonal influenza).
1 . A hemagglutination assay, comprising:
(a) mixing erythrocytes and a sample of an agglutinating virus under conditions permitting agglutination of the erythrocytes, and
(b) detecting hemagglutination in the mixture of (a) by light scattering.
2 . The hemagglutination assay of claim 1 , wherein the erythrocytes are human erythrocytes.
3 . The hemagglutination assay of claim 1 , wherein the erythrocytes are human group O erythrocytes.
4 . The hemagglutination assay of claim 1 , wherein the virus is an influenza virus A or an influenza virus B.
5 . The hemagglutination assay of claim 1 , wherein the detection of light scattering in (b) is performed using a bead array reader.
6 . The hemagglutination assay of claim 1 , wherein the sensitivity of said assay is increased at least about 10-fold in comparison to a hemagglutination assay wherein the detection of hemagglutination is by the human eye.
7 . The hemagglutination assay of claim 1 , wherein the assay is repeated with at least one two-fold dilution of the sample of virus.
8 . The hemagglutination assay of claim 1 , wherein the sensitivity of said assay is increased at least about 10-fold in comparison to a hemagglutination assay wherein the detection of hemagglutination is by the human eye and wherein the detection of light scattering in (b) is performed using a bead array reader.
9 . The hemagglutination assay of claim 1 , wherein the detected amount of hemagglutination in the mixture of (a) is less than about 10 erythrocytes.
10 . A hemagglutination inhibition assay, comprising:
(a) mixing a sample of an agglutinating virus and an antiserum under conditions permitting binding of an antibody in said antiserum to a hemagglutinin protein on the virus,
(b) mixing erythrocytes with the mixture of (a) under conditions permitting agglutination of the erythrocytes, and
(c) detecting hemagglutination in the mixture of (b) by light scattering.
11 . The hemagglutination inhibition assay of claim 10 , wherein the virus is an influenza virus A or an influenza virus B.
12 . The hemagglutination inhibition assay of claim 10 , wherein the antiserum is an antiserum that was raised against the virus.
13 . The hemagglutination inhibition assay of claim 10 , wherein the erythrocytes are human erythrocytes.
14 . The hemagglutination inhibition assay of claim 10 , wherein the erythrocytes are human group O erythrocytes.
15 . The hemagglutination inhibition assay of claim 10 , wherein the detection of light scattering in (c) is performed using a bead array reader.
16 . The hemagglutination inhibition assay of claim 10 , wherein the sensitivity of said assay is increased at least about 10-fold in comparison to a hemagglutination inhibition assay wherein the detection of hemagglutination is by the human eye.
17 . The hemagglutination inhibition assay of claim 10 , wherein the assay is repeated with at least one two-fold dilution of the sample of virus.
18 . The hemagglutination inhibition assay of claim 10 , wherein the assay is repeated with at least one two-fold dilution of the sample of antiserum.
19 . The hemagglutination inhibition assay of claim 10 , wherein the assay is repeated with at least one two-fold dilution of the sample of virus and at least one two-fold dilution of the sample of antiserum.
20 . The hemagglutination inhibition assay of claim 10 , wherein the sensitivity of said assay is increased at least about 10-fold in comparison to a hemagglutination inhibition assay wherein the detection of hemagglutination is by the human eye and wherein the detection of light scattering in (c) is performed using a bead array reader.
21 . The hemagglutination inhibition assay of claim 10 , wherein the detected amount of hemagglutination in the mixture of (b) is less than about 10 erythrocytes.
22 . A method of detecting hemagglutination in a sample, comprising detecting hemagglutination in a sample by light scattering.
23 . The method of claim 22 , wherein the detection of light scattering is performed using a bead array reader.
24 . The method of claim 22 , wherein the sensitivity of the detection is increased at least about 10-fold in comparison to a hemagglutination assay or a hemagglutination inhibition assay wherein the detection of hemagglutination is by the human eye.
25 . The method of claim 22 , wherein the detected amount of hemagglutination in the sample is less than about 10 erythrocytes.