CONJUGATED SMALL MOLECULES
Provided herein are linker compounds and conjugates that include the linker compounds. In one embodiment, the linker compounds comprise 2 or 3 residues of 6-aminohexanoic acid and optionally 7-10 residues of polyethyleneglycol (PEG). The linker compounds are useful in forming conjugates with one or more components useful in biopharmaceutical or bioanalytical applications. In particular, the biopharmaceutically useful compounds are kinase inhibitors. The conjugates described herein have utility in a variety of diagnostic, separation, and therapeutic applications.
1 . A compound having a formula:
A-(X 1 —(CR 1 R 2 ) p —C(═X 2 )) n —X 3 -T 1 -(CR 3 R 4 X 4 CR 5 R 6 ) m -T 2 -L-B (FORMULA A1)
wherein:
B is selected from:
A is biotin, a biotinyl group or a derivative of a biotinyl group;
X 1 , X 2 , X 3 , and X 4 are independently selected from the group consisting of O, S, and NH;
R 1 , R 2 , R 3 , R 4 , R 5 , R 6 , R 7 , and R 8 , are each independently selected from a group consisting of hydrogen, halogen, or lower alkyl;
T 1 and T 2 are each independently selected from a group consisting of (CH 2 ) r , a bond or triazole;
L is a bond or a moiety comprising a —NH— group;
p is 1, 2, 3, 4, 5, or 6;
n is 0, 1, 2, 3, 4, 5, or 6;
r is 1, 2, 3, 4, 5, or 6; and
m is 4 to 20.
2 . The compound of claim 1 , wherein L is a moiety comprising an —NH—C(O)— group.
3 . The compound of claim 1 , wherein L is selected from the group consisting of —NH—, —C(O)NH—, —NHC(O)NH—, and —C(O)CH 2 CH 2 C(O)NH—.
4 . The compound of claim 1 , wherein p is 5.
5 . The compound of claim 1 , wherein p is 5; n is 1, 2, or 3; and m is 6, 7, 8, 9, or 10.
6 . The compound of claim 1 , wherein A is:
7 . The compound of claim 1 , wherein X 2 is O.
8 . The compound of claim 1 , wherein X 4 is O.
9 . The compound of claim 1 , wherein T 2 is CH 2 .
10 . The compound of claim 1 , wherein T 1 is triazole.
11 . The compound of claim 1 selected from:
12 . A compound having formula: