Method for the analysis of circulating antibodies
View Patent ↗There is provided a method for the analysis of circulating antibodies comprising the steps: a) providing an analysis device comprising a substrate, and provided on said substrate at least one sample addition zone, at least one retaining zone, at least one sink, and at least one flow path connecting the sample addition zone, the retaining zone and the sink, wherein the flow path is open and comprises projections substantially vertical to the surface of said substrate and having a height (H), diameter (D) and reciprocal spacing (t 1 , t 2 ) such that lateral capillary flow of said sample is achieved and such that cells can flow through the projections, wherein said retaining zone comprises at least one affinity binding means to which cell structures are bound, b) adding at least one sample to a sample addition zone, and c) reading a result, wherein circulating antibodies directed against cell structures are determined.
1. A method for the analysis of circulating antibodies, said method comprising the steps of:
a. providing an analysis device comprising a substrate, and provided on said substrate at least one sample addition zone, at least one retaining zone, at least one sink, and at least one flow path connecting the at least one sample addition zone, the at least one retaining zone and the at least one sink, wherein the at least one flow path is open and comprises projections substantially vertical to the surface of said substrate and having a height (H), diameter (D) and reciprocal spacing (t 1 , t 2 ) such that lateral capillary flow of at least one liquid sample is spontaneously achieved along said at least one flow path and such that cells can flow through the projections, wherein said at least one retaining zone comprises at least one affinity binding means to which cell structures are bound,
b. binding cells or cell structures onto projections in said at least one retaining zone, said cells or cell structures forming binding surfaces,
c. adding a liquid sample to said sample addition zone,
d. binding circulating antibodies onto said formed binding surfaces, and
e. reading a result,
wherein circulating antibodies directed against bound cell structures are determined, in which the projections are defined by a tapered configuration and in which the projections are narrower at the top of the projections than the bottom and in which the reciprocal spacing between projections is larger between the top of adjacent projections than the bottom thereof.
2. The method according to claim 1 , wherein said at least one liquid sample is selected from the group consisting of human or animal blood, urine, lung liquids, synovial fluid, wound liquids, saliva, tears, and sweat.
3. The method according to claim 1 , wherein said at least one liquid sample is from human blood.
4. The method according to claim 1 , wherein said cell structures are part of the hematological antigen system.
5. The method according to claim 1 , wherein said cell structures are part of the antigens involved in HIV infection or detection.
6. The method according to claim 1 , wherein said at least one liquid sample is from human blood and is used for the determination of circulating antibodies directed against bacteria, viruses or small sized single or multi cell infectious agents.
7. The method according to claim 1 , wherein said at least one liquid sample is from human bone marrow.
8. The method according to claim 1 , wherein said affinity binding means is selected from at least one of an antibody, an aptamer, a receptor, a ligand, a single chain antibody, a fragmented antibody and a lectin.
9. The method according to claim 1 , wherein said cell structures are bound to said at least one retaining zone prior to said liquid sample adding step.
10. The method according to claim 1 , including a first liquid adding step and a second liquid adding step, in which cell structures are bound following said first liquid adding step and said antibodies are bound following said second liquid adding step.