Nucleic acids encoding a new family of chemokines, the CX3C family, from a mammal, reagents related thereto, including specific antibodies, and purified proteins are described. Methods of using said reagents and related diagnostic kits are also provided.
1. An isolated antibody or antigen-binding fragment thereof which specifically binds to a polypeptide wherein said polypeptide a) chemoattracts peripheral blood monocytes or T-cells, b) consists of an amino acid sequence comprising at least 95% sequence identity to the amino acid sequence set forth in SEQ ID NO: 8, and c) comprises a chemokine domain identical to residues 25 to 100 of the amino acid sequence set forth in SEQ ID NO: 8.
2. The antibody or antigen-binding fragment thereof of claim 1 , which is detectably labeled.
3. The antibody or antigen-binding fragment thereof of claim 1 , which is a polyclonal antibody.
4. The antibody or antigen-binding fragment thereof of claim 1 , which is a monoclonal antibody.
5. The antibody or antigen-binding fragment thereof of claim 1 , which is a Fab.
6. The antibody or antigen-binding fragment thereof of claim 1 , which is a F(ab) 2 .
7. The antibody or antigen-binding fragment thereof of claim 1 , which is sterile.
8. The antibody or antigen-binding fragment thereof of claim 1 , which is an antibody, wherein the antibody exhibits a Kd to said polypeptide of greater than about 100 nM.
9. The antibody or antigen-binding fragment thereof of claim 1 , which is an antibody, wherein the antibody exhibits a Kd to said polypeptide of greater than about 30 nM.
10. The antibody or antigen-binding fragment thereof of claim 1 , which is an antibody, wherein the antibody exhibits a Kd to said polypeptide of greater than about 10 nM.
11. The antibody or antigen-binding fragment thereof of claim 1 , which is an antibody, wherein the antibody exhibits a Kd to said polypeptide of greater than about 3 nM.
12. A kit comprising the antibody or antigen-binding fragment thereof of claim 1 , and: a) instructional material for the antibody or antigen-binding fragment thereof; or b) a container into which the antibody or antigen-binding fragment thereof is segregated; or c) both a) and b).
13. A complex comprising an antibody or antigen-binding fragment thereof of claim 1 bound to said polypeptide.
14. A pharmaceutical composition comprising the antibody or antigen-binding fragment of claim 1 and a pharmaceutically acceptable carrier.
15. An isolated antibody or antigen-binding fragment thereof which specifically binds to a polypeptide consisting of an amino acid sequence set forth in SEQ ID NO: 8.
16. The antibody or antigen-binding fragment thereof of claim 15 , wherein said polypeptide is denatured.
17. An isolated antibody or antigen-binding fragment thereof, that specifically binds to a polypeptide consisting of Gln25 to Gly100 of the amino acid sequence set forth in SEQ ID NO: 8.
18. The antibody or antigen-binding fragment thereof of claim 17 that specifically binds to a polypeptide consisting of Gln25 to Gly100 of the amino acid sequence set forth in SEQ ID NO: 8, wherein the antibody or fragment is raised against a purified or recombinantly produced polypeptide comprising Gln25 to Gly100 of the amino acid sequence set forth in SEQ ID NO: 8.
19. The antibody or antigen-binding fragment thereof of claim 18 that specifically binds to a polypeptide consisting of Gln25 to Gly100 of the amino acid sequence set forth in SEQ ID NO: 8, wherein the antibody or fragment is raised against a purified or recombinantly produced polypeptide comprising a mature polypeptide comprising the amino acid sequence set forth in SEQ ID NO: 8.
20. A method for detecting a polypeptide in a biological sample, comprising: a) contacting the biological sample with an antibody or antigen-binding fragment thereof of claim 1 under conditions to permit formation of an antibody or antigen-binding fragment thereof: polypeptide complex; and b) detecting the complex.
21. The method of claim 20 , wherein the biological sample is human.
22. An isolated monoclonal antibody that binds specifically to a polypeptide consisting of amino acids 25-397 of SEQ ID NO: 8.
23. A pharmaceutical composition comprising an isolated monoclonal antibody that binds specifically to a polypeptide consisting of amino acids 25-397 of SEQ ID NO: 8 and a pharmaceutically acceptable carrier.