Biocompatible phase invertible proteinaceous compositions and methods for making and using the same
View Patent ↗Biocompatible phase invertible proteinaceous compositions and methods for making and using the same are provided. The subject phase invertible compositions are prepared by combining a proteinaceous substrate and a crosslinking agent, such as a stabilized aldehyde crosslinking agent. Also provided are kits for use in preparing the subject compositions. The subject compositions, kits and systems find use in a variety of different applications.
1. A crosslinking composition produced by maintaining an initial dialdehyde composition at a temperature ranging from about 45 to about 55° C. for a period of time ranging from about 1 to about 20 days.
2. The crosslinking composition according to claim 1 , wherein the initial dialdehyde is glutaraldehyde.
3. The crosslinking composition according to claim 2 , wherein the crosslinking composition comprises molecules described by the following formula:
wherein R 1 and R 2 are H or hydrocarbon moieties which are polymerization products produced by sequential aldol reaction and dehydration of one or more glutaraldehyde molecules.
4. The crosslinking composition according to claim 3 , wherein the crosslinking composition comprises crosslinking molecules described by the formula:
5. The crosslinking composition according to claim 3 , wherein upon combination with a proteinaceous substrate the crosslinking composition produces a pyridinium moiety-containing crosslinking structure.
6. A kit comprising:
(a) a substrate comprising a proteinaceous substrate; and
(b) a crosslinking composition produced by maintaining an initial glutaraldehyde composition at a temperature ranging from about 45 to about 55° C. for a period of time ranging from about 1 to about 20 days.
7. The kit according to claim 6 , wherein the crosslinking composition comprises molecules described by the following formula:
wherein R 1 and R 2 are H or hydrocarbon moieties which are polymerization products produced by sequential aldol reaction and dehydration of one or more glutaraldehyde molecules.
8. The kit according to claim 7 , wherein the crosslinking composition comprises molecules described by the formula:
9. The kit according to claim 7 , wherein upon combination with a proteinaceous substrate the crosslinking composition produces a pyridinium moiety-containing crosslinking structure.
10. The kit according to claim 6 , wherein the proteinaceous substrate comprises one or more of: albumin, elastin, fibrin and soluble and insoluble forms of collagen and combinations thereof.
11. The kit according to claim 6 , wherein said substrate further comprises an adhesion modifier.
12. The kit according to claim 6 , wherein said substrate further comprises a plasticizer.
13. The kit according to claim 6 , wherein the substrate further comprises a carbohydrate.
14. The kit according to claim 6 , wherein said substrate further comprises a filler.
15. The kit according to claim 6 , wherein said substrate further comprises a polyblend.
16. The kit according to claim 6 , wherein said substrate further comprises a biologically active agent.
17. The kit according to claim 6 , wherein said substrate further comprises a foaming agent.
18. The kit according to claim 6 , wherein the kit further comprises a delivery device for delivering a phase invertible composition produced by combining the substrate and the crosslinking composition.
19. A phase invertible composition produced by combining:
(a) a substrate comprising a proteinaceous substrate; and
(b) a crosslinking composition produced by maintaining an initial dialdehyde composition at a temperature ranging from about 45 to about 55° C. for a period of time ranging from about 1 to about 20 days,
wherein the crosslinking composition produces a pyridinium moiety-containing crosslinking structure upon combination with the proteinaceous substrate.
20. The phase invertible composition according to claim 19 , wherein the initial dialdehyde is glutaraldehyde.