Wound dressing compositions
View Patent ↗A wound dressing composition comprising a non-viable cell lysate or releasate derived from a hepatocyte cell or an inflammatory cell such as a macrophage. Also provided are wound dressings comprising such compositions, methods of making such compositions, and the use of such compositions for the treatment of wounds.
1. A wound dressing composition comprising a non-viable whole cell lysate, cell lysate fraction or releasate derived from a hepatocyte cell, wherein the whole cell lysate, cell lysate fraction or releasate does not include intact organelles.
2. The wound dressing composition according to claim 1 , wherein the cell lysate, cell lysate fraction or releasate is dispersed in or on a pharmaceutically acceptable vehicle.
3. The wound dressing composition according to claim 2 , wherein said pharmaceutically acceptable vehicle is in the form of a solid sheet, a semi-solid ointment, an apertured solid sheet, a web, a woven fabric, a knitted fabric, a nonwoven fabric, a hydrophilic foam, a freeze-dried sponge or a solvent-dried sponge.
4. The wound dressing composition according to claim 3 , wherein said pharmaceutically acceptable vehicle comprises a bioabsorbable freeze-dried sponge.
5. The wound dressing composition according to claim 1 , wherein the cell releasate, cell lysate fraction or whole cell lysate is dispersed in or on a solid vehicle that comprises a solid bioabsorbable material selected from the group consisting of collagen, chitosan, oxidized cellulose, or mixtures thereof.
6. The wound dressing composition according to claim 1 which is substantially dry.
7. The wound dressing composition according to claim 1 , wherein the composition further comprises from about 0.01 to about 10% by weight on a dry weight basis of one or more wound healing therapeutic substances.
8. The wound dressing composition according to claim 1 which is substantially sterile.
9. A wound dressing comprising a wound dressing composition according to claim 1 .
10. The wound dressing according to claim 9 which is sterile and packaged in a microorganism-impermeable container.
11. A method of preparing a medicament for the treatment of a wound comprising the steps of:
providing a non-viable whole cell lysate, cell lysate fraction or releaseate derived from a hepatocyte cell; and
dispersing the lysate cell lysate fraction or releaseate in or on a pharmaceutically acceptable vehicle.
12. The method according to claim 11 , wherein the we is a chronic wound.
13. The method according to claim 12 , wherein said chronic wound is selected from the group consisting of venous ulcers, decubitis ulcers and diabetic ulcers.
14. A wound dressing composition comprising a non-viable whole cell lysate derived from a hepatocyte cell, wherein the concentration of protease inhibitors in the cells of the non-viable whole cell lysate is greater than a basal level, or a pellet fraction obtained by subjecting the whole cell lysate to centrifugation, or a supernatant fraction obtained by subjecting the whole cell lysate to centrifugation, or a sub-fraction of the whole cell lysate.
15. The wound dressing composition of claim 14 , wherein the whole cell lysate does not include intact organelles.
16. The wound dressing composition of claim 14 , wherein the sub-fraction of the whole cell lysate is a releasate.
17. The wound dressing composition claim 14 , wherein at least one of the protease inhibitors is alpha-1-antitrypsin, and wherein the concentration of alpha-1-antitrypsin is greater than the basal level.
18. The wound dressing composition of claim 14 , comprising a non-viable whole cell lysate derived from a hepatocyte cell, or a pellet fraction obtained by subjecting the whole cell lysate to centrifugation, or a supernatant fraction obtained by subjecting the whole cell lysate to centrifugation.
19. The wound dressing composition of claim 1 , comprising a on-viable whole cell lysate or cell lysate fraction derived from a hepatocyte cell.
20. The wound dressing composition of claim 1 , comprising a non-viable whole cell lysate derived from a hepatocyte cell.