METHOD FOR IN SITU SOLIDIFICATION OF BLOOD-POLYMER COMPOSITIONS FOR REGENERATIVE MEDICINE AND CARTILAGE REPAIR APPLICATIONS
The present invention relates to a method for repairing or regenerating tissues in a patient such as cartilage, meniscus, ligament, tendon, bone, skin, cornea, periodontal tissues, abscesses, resected tumors, cardiac tissues and ulcers. The method comprises the step of administering simultaneously or sequentially a pro-coagulant factor and an effective amount of a polymer composition comprising a biocompatible polymer and blood or a component thereof. When the polymer composition is in contact with the pro-coagulant factor it is converted into a non-liquid state such that the polymer composition will adhere to the site in need of repair to effect repair of the tissue and/or regeneration thereof.
1 . A method for repair and/or regeneration of a tissue of a patient comprising administering into said tissue in need of repair and/or regeneration:
i. a pro-coagulant factor; and
ii. a polymer composition comprising:
a biocompatible polymer; and
blood or a blood component thereof,
wherein the polymer composition in contact with the pro-coagulant factor is converted into a non-liquid state such that the polymer composition when placed at the site in need of repair will adhere to the site in need of repair to effect repair and/or regeneration of the tissue.
2 . The method of claim 1 , wherein the biocompatible polymer is selected from the group consisting of a polysaccharide, a protein, and a polyamino acid.
3 . The method of claim 2 , wherein the polysaccharide is selected from the group consisting of chitosan, chitin, hyaluronan, glycosaminoglycan, chondroitin sulfate, keratan sulfate, dermatan sulfate, heparin, cellulose, and heparin sulfate.
4 . The method of claim 2 , wherein the polysaccharide is chitosan.
5 .- 6 . (canceled)
7 . The method of claim 1 , wherein the blood or blood component thereof is autologous to the patient.
8 . (canceled)
9 . The method of claim 1 , wherein the blood component comprises platelet rich plasma.
10 . (canceled)
11 . The method of claim 1 , wherein the polymer composition further comprises a growth factor or a chemotactic factor.
12 .- 16 . (canceled)
17 . The method of claim 1 , wherein the pro-coagulant factor is selected from the group consisting of a clotting factor, a platelet-activating factor, collagen, ellagic acid, epinephrine and adenosine diphosphate.
18 .- 23 . (canceled)
24 . A method for repair and/or regeneration of a tissue of a patient comprising administering into said tissue in need of repair and/or regeneration:
i. a pro-coagulant factor; and
ii. a polymer composition comprising:
chitosan;
glycerol phosphate; and
blood or blood component thereof,
wherein the polymer composition is converted into a non-liquid state, such that said polymer composition once converted into a non-liquid state adheres to the site in need of repair and/or regeneration when placed thereon to effect repair and/or regeneration of the tissue.
25 . (canceled)
26 . The method of claim 24 , wherein the blood or component thereof is autologous to the patient.
27 . (canceled)
28 . The method of claim 24 , wherein the blood component comprises platelet rich plasma.
29 . (canceled)
30 . The method of claim 24 , wherein the polymer composition further comprises a growth factor or a chemotactic factor.
31 .- 34 . (canceled)
35 . The method of claim 24 , wherein the pro-coagulant factor is selected from the group consisting of a clotting factor, a platelet-activating factor, collagen, ellagic acid, epinephrine and adenosine diphosphate.
36 .- 37 . (canceled)
38 . The method of claim 24 , wherein administering of the pro-coagulant factor to the tissue is done either simultaneously, immediately prior to, or with a delay of up to 2 minutes, before administering the polymer composition, or immediately after, or with a delay of up to 2 minutes, administering the polymer composition.
39 .- 41 . (canceled)
42 . A kit for repair and/or regeneration of a tissue of a patient, said kit comprising i) a pro-coagulant factor and ii) a polymer composition comprising a biocompatible polymer.
43 . (canceled)
44 . The kit of claim 42 , further comprising blood or a blood component thereof.
45 . The kit of claim 42 , further comprising cells or additional bioactive factors.
46 . The kit of claim 42 , wherein the biocompatible polymer is selected from the group consisting of a polysaccharide, a protein, and a polyamino acid.
47 . The kit of claim 46 , wherein the polysaccharide is selected from the group consisting of chitosan, chitin, hyaluronan, glycosaminoglycan, chondroitin sulfate, keratan sulfate, dermatan sulfate, heparin, cellulose, and heparin sulfate.
48 . The kit of claim 46 , wherein the polysaccharide is chitosan.
49 .- 50 . (canceled)
51 . The kit of claim 44 , wherein the blood or component thereof is autologous to the patient.
52 . (canceled)
53 . The kit of claim 44 , wherein the blood component comprises platelet rich plasma.
54 . (canceled)
55 . The kit of claim 42 , further comprising a growth factor or a chemotactic factor.
56 . (canceled)
57 . The kit of claim 42 , wherein the pro-coagulant factor is selected from the group consisting of a clotting factor, a platelet-activating factor, collagen, ellagic acid, epinephrine and adenosine diphosphate.
58 .- 62 . (canceled)
63 . A kit for repair and/or regeneration in a tissue of a patient comprising i) a pro-coagulant factor and ii) a polymer composition comprising chitosan and glycerol phosphate.
64 . The kit of claim 63 , further comprising blood or a component of said blood.
65 .- 67 . (canceled)
68 . The kit of claim 64 , wherein the blood component comprises platelet rich plasma.
69 . The kit of claim 63 , further comprising a growth factor or a chemotactic factor.
70 . (canceled)
71 . The kit of claim 63 , wherein the pro-coagulant factor is selected from the group consisting of a clotting factor, a platelet-activating factor, collagen, ellagic acid, epinephrine and adenosine diphosphate.
72 .- 120 . (canceled)