Compositions of human lipids and methods of making and using same
The disclosure features methods of making compositions that include a human lipid. The methods can include: obtaining whole human milk; separating the milk into a cream portion and a skim portion; processing the cream portion; and pasteurizing the processed cream portion.
1. A method of making a composition comprising a human lipid, the method comprising:
(a) obtaining whole human milk;
(b) separating the milk into a cream portion and a skim portion;
(c) filtering the water out of the skim portion after step (b);
(d) suspending the cream portion obtained in step (b) with the water obtained in step (c);
(e) purifying the suspended cream portion obtained in step (d) to obtain a composition comprising a human lipid; and
(f) parenterally administering the composition comprising a human lipid obtained in step (e) to a human subject.
2. The method of claim 1 , further comprising
(i) separating the cream portion obtained in step (b) into a second cream portion and a second skim portion; and
(ii) in step (d) suspending both the cream portion and the second cream portion with the water obtained in step (c).
3. The method of claim 1 , further comprising pasteurizing the composition comprising a human lipid after step (e).
4. The method of claim 1 , wherein the purifying comprises diafiltration.
5. The method of claim 4 , further comprising ultrafiltrating.
6. The method of claim 1 , further comprising adding one or more constituents to the composition obtained in step (e) selected from the group consisting of: an emulsifier, a preservative, a diluent, and an osmolality modifier.
7. The method of claim 6 , wherein the preservative is an antioxidant.
8. The method of claim 7 , wherein the antioxidant is selected from the group consisting of: alpha-tocopherol, vitamin C, a carotenoid, and a retinoid.
9. The method of claim 6 , wherein the osmolality modifier comprises glycerin.
10. The method of claim 1 , further comprising, adding one or more constituents to the composition obtained in step (e) selected from the group consisting of: a vitamin, a mineral, and a carbohydrate.
11. The method of claim 1 , wherein the administration is part of total parenteral nutritional administration.
12. The method of claim 1 , wherein the subject is a premature infant.
13. The method of claim 1 , wherein the subject has a nutritional disease or disorder.