Device and method for precipitation of peptides
View Patent ↗The present invention relates to a method for precipitation of peptide where the mixing step of the peptide with the precipitation aid and the precipitation itself are specially separated.
1. A method for the precipitation of peptides, comprising the steps:
a) a first mixing step of an aqueous peptide solution with a precipitation aid, whereby an average mixing velocity of >0.05 m/s and <5 m/s is achieved in this step using a jet mixer; and
b) a precipitation step in order to precipitate the peptide;
whereby steps a) and b) are carried out spacially separated from each other.
2. The method according to claim 1 , whereby in step a) [Delta]p is >0.05 bar and <20 bar.
3. The method according to claim 1 , whereby the macroscopic mixing time in step a) is >1 ms and <1000 ms.
4. The method according to claim 1 , whereby the macroscopic mixing time in step a) is >8 ms and <120 ms.
5. The method according to claim 1 , whereby step b) is performed while continously agitating with a blade agitator.
6. The method according to claim 1 , whereby step b) is performed while agitating with >0.1 W and <50 W.
7. The method according to claim 1 , whereby the agitator is set with an extrentricity of >0 and <0.035.
8. The method according to claim 1 , whereby the ratio of the blade diameter d to the diameter of the vessel and/or the compartment where step b) is performed is d:D >0.4:1 and <0.7:1.
9. The method according to claim 1 , whereby the ratio of the vessel and/or the compartment where step a) is performed towards the vessel and/or the compartment where step b) is performed is >0.02:1 and <0.08:1.
10. The method according to claim 1 , comprising a step a1) between steps a) and b): a1) performing a first precipitation and/or heat exchange in order to improve the precipitation and remove heat of mixing of step a).
11. The method according to claim 1 , comprising a step c) after steps a) and b): c) constantly and/or discontinuously delivering a part of the solution of the precipitation suspension out of step b) to be co-admixed in step a).
12. The method according to claim 1 , comprising a step a2) and a3) between steps a1) and b): a2) admixing further precipitation aid and a3) optionally repeating steps a1) and a2) ad lib.
13. A device for carrying out the method according to claim 1 .