Generation of oncolytic adenoviruses and uses thereof
View Patent ↗The present invention relates to a method for the production of onolytic adenoviruses having increased potency and their therapeutic applications for cancer. Recombinant adenoviruses and methods to produce them are provided.
1. An oncolytic isolated adenovirus which comprises a nucleotide sequence selected from the group consisting of SEQ ID NO:1 and SEQ ID NO:2.
2. The adenovirus of claim 1 , wherein the adenovirus comprises SEQ ID NO:1.
3. The adenovirus of claim 2 , wherein the adenovirus comprises SEQ ID NO:2.
4. The adenovirus of claim 1 , further comprising a heterologous gene, wherein said heterologous gene is expressed within a cell infected with said adenovirus.
5. The adenovirus of claim 4 , wherein said heterologous gene is thymidine kinase.
6. The adenovirus of claim 4 , wherein said heterologous gene encodes a therapeutic protein selected from the group consisting of cytokines and chemokines, antibodies, factors known to induce cell death, pro-drug converting enzymes and immunoregulatory proteins.
7. The oncolytic adenovirus of claim 1 , further comprising a heterologous gene encoding an immunoregulatory protein.
8. A method of inhibiting growth of a cancer cell, comprising infecting said cancer cell with the oncolytic adenovirus of claim 1 .
9. The method of claim 8 , wherein said cancer cell is an ovarian cancer cell.
10. The method of claim 9 , wherein said cancer cell is a drug-resistant ovarian cancer cell.
11. The method of claim 8 , wherein the adenovirus comprises SEQ ID NO:1.
12. The method of claim 8 , wherein the adenovirus comprises SEQ ID NO:2.