METHODS FOR INCREASING LEVELS OF HUMAN FETAL HEMOGLOBIN
The invention provides methods for increasing the level of human fetal hemoglobin in a subject or cell in need thereof. The methods can be used with subjects suffering from a β-chain hemoglobinopathy including thalassemia (e.g., β-thalassemia) or sickle cell anemia.
1 . A method of increasing the level of human fetal hemoglobin in a subject, the method comprising:
administering a therapeutically effective amount of romidepsin to a subject in need of increased levels of human fetal hemoglobin.
2 . The method of claim 1 , wherein romidepsin is of the formula:
3 . The method of claim 1 , wherein the subject has a β-chain hemoglobinopathy.
4 . The method of claim 1 , wherein the subject has thalassemia.
5 . The method of claim 1 , wherein the subject has sickle cell anemia.
6 . The method of claim 1 , wherein the romidepsin is administered orally.
7 . The method of claim 1 , wherein the romidepsin is administered intravenously.
8 . The method of claim 1 , wherein the therapeutically effective amount of romidepsin ranges from 1 mg/m 2 to 150 mg/m 2 .
9 . The method of claim 6 , wherein the therapeutically effective amount of romidepsin ranges from 75 mg/m 2 to 100 mg/m 2 .
10 . The method of claim 7 , wherein the therapeutically effective amount of romidepsin ranges from 5 mg/m 2 to 25 mg/m 2 .
11 . The method of claim 10 , wherein the therapeutically effective amount of romidepsin ranges from 10 mg/m 2 to 20 mg/m 2 .
12 . The method of claim 11 , wherein the therapeutically effective amount of romidepsin is 14 mg/m 2 .
13 . The method of claim 1 , wherein the subject is a human.
14 . A method of increasing the level of human fetal hemoglobin in a cell, the method comprising:
administering an amount of romidepsin effective to increase the level of human fetal hemoglobin in a cell.
15 . The method of claim 14 , wherein the cell is a cell that produces hemoglobin.
16 . The method of claim 14 , wherein the concentration of romidepsin ranges from approximately 0.0185 nM to approximately 18.5 nM.
17 . The method of claim 14 , wherein the concentration of romidepsin ranges from approximately 0.185 nM to approximately 1.85 nM.
18 . The method of claim 14 , wherein the cell is in culture.
19 . The method of claim 14 , wherein the cell is within an animal.