IP Library › Granted Patent US 8,580,935
Granted Patent B2
US 8,580,935 · App. 12/528,670 · Granted Nov 12, 2013

Ultra-fast chromatography

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Quick Facts
Patent No.
US 8,580,935
App. No.
12/528,670
Granted
Nov 12, 2013
Kind
B2
Abstract

The present invention relates to a chromatographic method of separating biological material comprising, providing chromatographic media comprising inorganic oxide particles having an average diameter of about 2 microns or less and an average pore diameter of 300 Å or more; applying a solvent comprising said biological material to said media, wherein said biological material is reversibly bonded to said media; and eluting said biological material from said media with a solvent in less than about 2 minutes for biological material having a molecular weight of less than about 100,000 Daltons.

Claims (11)

1. A chromatographic method of separating biological material comprising:

(a). providing a chromatographic medium comprising inorganic oxide particles having an average diameter of 2 microns or less and an average pore diameter of 300 Å or more;

(b) applying a first solvent comprising said biological material to said medium, wherein said biological material is reversibly bonded to said medium; and

(c) eluting said biological material from said medium with a second solvent in 2 minutes or less, wherein each component in the biological material has a molecular weight of 100,000 Daltons or less.

2. The method according to claim 1 , wherein said biological material comprises one or more proteins and peptides.

3. The method according to claim 1 , wherein said biological material comprises one or more proteins.

4. The method according to claim 1 , wherein said inorganic oxide particles have an average particle size ranging from about 1.0 μm to about 2.0 μm.

5. The method according to claim 1 , wherein said solvent comprises water, acetonitrile, and trifluoroacetic acid.

6. The method according to claim 1 , wherein said solvent comprises 0.08 to 0.1% v/v trifluoroacetic acid in water and acetonitrile.

7. The method according to claim 1 , wherein said eluting of said biological material from said medium occurs in 1.5 minutes or less.

8. The method according to claim 1 , wherein said eluting of said biological material from said medium occurs in 1.2 minutes or less.

Assignments (3)
RELEASE OF SECURITY AGREEMENT RECORDED AT REEL/FRAME NO.: 032157/0264 Recorded Apr 3, 2018
From: GOLDMAN SACHS BANK USA, AS THE COLLATERAL AGENT
To: ALLTECH ASSOCIATES, INC.
Reel/Frame 045832/0804 →
ASSIGNMENT OF ASSIGNOR'S INTEREST Recorded Jul 12, 2017
From: ALLTECH ASSOCIATES, INC.
To: W. R. GRACE & CO.-CONN.
Reel/Frame 042983/0417 →
SECURITY AGREEMENT Recorded Feb 4, 2014
From: ALLTECH ASSOCIATES, INC.
To: GOLDMAN SACHS BANK USA, AS THE COLLATERAL AGENT
Reel/Frame 032157/0264 →