Method for production of tooth, and tooth produced by the method
View Patent ↗Disclosed is a method for producing a tooth, which comprises the steps of: positioning a first cell mass substantially comprising either one of amniotic mesenchymal cells or epithelial cells, and a second cell mass substantially comprising the other one in the inside of a support carrier while keeping them in close contact with each other without being mixed together; and culturing the first and second cell masses in the inside of the support carrier.
1. A method for producing a tooth, the method comprising:
isolating or obtaining mesenchymal cells from an amnion and preparing a first cell mass consisting essentially of the mesenchymal cells;
isolating or obtaining epithelial cells from a tooth germ and preparing a second cell mass consisting essentially of the epithelial cells;
positioning said first and second cell masses in a support carrier capable of promoting tooth formation, wherein the first and second cell masses are not mixed but placed in close contact with each other within the support carrier; and
culturing the first and second cell masses in the support carrier for an amount of time sufficient to produce a tooth.
2. The method according to claim 1 , wherein said isolating or obtaining mesenchymal cells from an amnion comprises isolating or obtaining mesenchymal cells from an amniotic compact layer of said anion.
3. The method according to claim 1 , wherein said first cell mass is composed of single cells.
4. The method according to claim 1 , wherein said first cell mass and second cell mass are both composed of single cells.
5. The method according to claim 1 , wherein said support carrier is at least one selected from the group consisting of collagen, fibrin, laminin, an extracellular matrix mixture, polyglycolic acid (PGA), polylactic acid (PLA), and lactic acid/glycolic acid copolymer (PLGA).
6. The method according to claim 1 , further comprising continuing said culturing step until a tooth and periodontal tissue are formed.
7. The method according to claim 1 , wherein multiple teeth are formed simultaneously after said culturing, thereby resulting in a complex of teeth.
8. A method for transplanting a tooth, the method comprising:
obtaining a complex of teeth prepared by the method according to claim 7 ;
separating each tooth from the complex of teeth to produce a separated tooth; and
transplanting said separated tooth into a transplantation site comprising other teeth and aligning said separated tooth with said other teeth present at said transplantation site.
9. A method for treating a lost tooth or replacing a damaged tooth in a subject, the method comprising:
transplanting a tooth and/or periodontal tissue obtained by the method according to claim 6 into a site of a lost tooth or an extracted, damaged tooth in a subject.
10. The method according to claim 1 , wherein the support carrier is a gel support carrier and wherein said first cell mass is positioned in the gel support carrier before positioning said second cell mass in the gel support carrier.
11. The method according to claim 10 , wherein the gel support carrier is a collagen gel support carrier.
12. The method according to claim 10 , further comprising:
solidifying the gel after the second cell mass is brought into close contact with the first cell mass.