Synthetic cell platforms and methods of use thereof
The present invention provides synthetic cell platforms. The synthetic cell platforms can be used for culturing cells in vitro. The synthetic cell platforms can also be implanted together with bound cells into an individual. The present invention provides methods of using the platforms to provide cells or progeny of such cells for use in various applications, including clinical applications; and methods of use of the platforms to introduce cells into an individual.
1. A synthetic cell platform comprising:
a) a synthetic substrate; and
b) a mammalian stem cell-binding peptide covalently linked to the synthetic substrate, wherein the stem cell binding peptide has a length of up to about 50 amino acids, and wherein the stem cell binding peptide comprises the amino acid sequence SEQ ID NO: 22.
2. The synthetic cell platform of claim 1 , wherein the stem cell-binding peptide is covalently linked to the synthetic substrate at a density of from about 0.1 pmol/cm 2 to about 100 pmol/cm 2 .
3. The synthetic cell platform of claim 1 , wherein the stem cell-binding peptide is covalently linked to the synthetic substrate at a density of from about 1 pmol/cm 2 to about 25 pmol/cm 2 .
4. The synthetic cell platform of claim 1 , wherein the synthetic substrate is an interpenetrating polymer network, a synthetic hydrogel, a semi-interpenetrating polymer network, or a thermo-responsive polymer.
5. The synthetic cell platform of claim 1 , wherein the synthetic substrate comprises a co-polymer of polyacrylamide and poly(ethylene glycol).
6. The synthetic cell platform of claim 1 , wherein the stem cell is an embryonic stem cell, a neural stem cell, a hematopoietic stem cell, a mesenchymal stem cell, or an induced pluripotent stem cell.
7. The synthetic cell platform of claim 1 , further comprising a mammalian stem cell bound to the peptide.