OPTOINJECTION METHODS
Optoinjection method for transiently permeabilizing a target cell by (a) illuminating a population of cells contained in a frame; (b) detecting at least one property of light directed from the frame; (c) locating a target cell by the property of light; and (d) irradiating the target cell with a pulse of radiation.
1 . A method for transiently permeabilizing a target cell, comprising the steps of:
(a) illuminating a population of substantially stationary cells contained in a frame;
(b) obtaining a static representation of at least one property of light directed simultaneously from the frame;
(c) locating a target cell in the population of cells, wherein the target cell is located with reference to the static representation; and
(d) irradiating the target cell with a pulse of radiation,
whereby the target cell is transiently permeabilized.