IMMUNOLOGICAL COMPOSITION
The present invention relates to immunological compositions comprising sulpholipo-cyclodextrin (SL-CD) and, saponin or Quil A, and optionally at least one antigen. The invention relates to methods and immunological compositions comprising at least one antigen, which may be a veterinary antigen. The veterinary antigen in the methods and immunological compositions of the invention may be a bovine antigen. The invention relates to methods and immunological compositions comprising bovine ephemeral fever virus (BEFV), bovine herpesvirus 1 (IBR) or bluetongue virus (BTV). The invention comprises methods for eliciting an immune response against BEFV, IBR, or BTV in an animal, which comprises administering to the animal a composition of the invention. In the invention, particularly the immune response is a protective immune response. The invention comprises a method for preparing an immunological composition comprising adding Quil A to a virus.
1 . An immunological composition comprising sulpholipo-cyclodextrin (SL-CD) and saponin.
2 . The immunological composition of claim 1 further comprising at least one antigen.
3 . The immunological composition of claim 2 , wherein the at least one antigen is selected from bacteria, viruses, peptides, polypeptides, nucleic acids, or combinations thereof.
4 . The immunological composition of claim 3 , wherein the at least one antigen is a veterinary antigen.
5 . The immunological composition of claim 4 , wherein the at least one antigen is a bovine antigen.
6 . The immunological composition of claim 3 , wherein the at least one antigen is a viral antigen.
7 . The immunological composition of claim 6 , wherein the viral antigen is bovine ephemeral fever virus (BEFV); bovine herpes virus 1 (IBR), or bluetongue virus (BTV).
8 . The immunological composition of claim 7 wherein the SL-CD is present at a final concentration of about 0.2 mL/mL.
9 . The immunological composition of claims 7 wherein the saponin is present at a final concentration of about 0.5 mg/mL.
10 . The immunological composition of claim 7 wherein the saponin is Quil A.
11 . The immunological composition of claim 10 wherein the Quil A is present at a final concentration of about 0.1 mg/mL to about 0.2 mg/mL.
12 . The immunological composition of claim 11 wherein the Quil A is present at a final concentration of about 0.158 mg/mL.
13 . A method for eliciting an immune response in an animal in need thereof, which comprises administering to the animal the immunological composition of claim 8 wherein the saponin is present at a final concentration of about 0.5 mg/mL, or wherein the saponin is Quil A, and the Quil A is present at a final concentration of about 0.1 mg/mL to about 0.2 mg/mL, or the Quil A is present at a final concentration of about 0.158 mg/mL.
14 . An immunological composition prepared by combining Quil A and a viral antigen prior to adding at least one additional adjuvant.
15 . The immunological composition prepared as in claim 14 , further comprising at least one additional adjuvant.
16 . The immunological composition prepared as in claim 15 , wherein the at least one additional adjuvant is selected from SL-CD, aluminum hydroxide, SP-oil, or carbopol.
17 . The immunological composition prepared as in claim 16 , wherein the at least one additional adjuvant is SL-CD.
18 . The immunological composition prepared as in claim 17 wherein the SL-CD is present at a final concentration of about 0.2 mL/mL.
19 . The immunological composition prepared as in claim 14 wherein the Quil A is present at a final concentration of about 0.1 mg/mL to about 0.2 mg/mL.
20 . The immunological composition prepared as in claims 14 wherein the Quil A is present at a final concentration of about 0.158 mg/mL.
21 . The immunological composition prepared as in claim 14 wherein the viral antigen is a bovine antigen.
22 . The immunological composition prepared as in claim 21 wherein the viral antigen is BEFV, IBR, or BTV.
23 . A method for eliciting an immune response against BEFV in an animal which comprises administering to the animal the composition according to claim 22 .
24 . The method of claim 23 wherein the immune response is elicited after administration of a single dose of the composition.
25 . The method of claim 23 wherein the immune response is a protective immune response.
26 . A kit for eliciting an immune response in an animal, comprising SL-CD and saponin, or SL-CD and Quil A
27 . The kit of claim 26 , further comprising at least one antigen.
28 . The kit of claim 27 , wherein the at least one antigen is selected from bacteria, viruses, peptides, polypeptides, nucleic acids, or combinations thereof.
29 . The kit of claim 28 , wherein the at least one antigen is a viral antigen.
30 . The kit of claim 29 , wherein the viral antigen is selected from BEFV, IBR, and BTV.