SYSTEMS AND METHODS FOR PRODUCTION OF MIXED FATTY ESTERS

1 . A method of producing a fatty acid methyl ester comprising:

providing a fatty ester production host,

providing methanol to the fatty ester production host,

and converting the methanol to a fatty acid methyl ester using the fatty ester production host.

2 . The method of claim 1 , wherein the fatty ester production host comprises a heterologous nucleic acid sequence encoding an ester synthase.

3 . The method of claim 2 , wherein the heterologous nucleic acid sequence encoding the ester synthase is: atfA1,wax-dgat, or mWS.

4 . The method of claim 1 , wherein the fatty ester production host comprises a heterologous nucleic acid sequence encoding a thioesterase.

5 . The method of claim 4 , wherein the heterologous nucleic acid sequence encoding the thioesterase is: tesA, ‘tesA, tesB, fatB, fatB2, fatB3, fatB [M141T], fatA, or fatA1.

6 . The method of claim 1 , wherein the fatty ester production host comprises a heterologous nucleic acid sequence encoding an acyl-CoA synthase.

7 . The method of claim 6 , wherein the heterologous nucleic acid sequence encoding the acyl-CoA synthase is: fadD, fadK, BH3103, yhfL, Pfl-4354, EAV15023, fadD1, fadD2, RPC — 4074, fadDD35, fadDD22, faa3p, or a gene encoding ZP — 01644857.

8 . The method of claim 1 , wherein the fatty ester production host either lacks a nucleic acid sequence encoding for an acyl-CoA dehydrogenase or expresses an attenuated level of an acyl-CoA dehydrogenase.

9 . The method of claim 1 , wherein the fatty ester production host is selected from the group consisting of at least one of the following: a mammalian cell, plant cell, insect cell, yeast cell, fungus cell, filamentous fungi cell, bacterial cell, a Gram-positive bacteria, a Gram-negative bacteria, the genus Escherichia, the genus Bacillus, the genus Lactobacillus, the genus Rhodococcus, the genus Pseudomonas, the genus Aspergillus, the genus Trichoderma, the genus Neurospora, the genus Fusarium, the genus Humicola, the genus Rhizomucor, the genus Kluyveromyces, the genus Pichia, the genus Mucor, the genus Myceliphtora, the genus Pencicillium, the genus Phanerochaete, the genus Pleurotus, the genus Trametes, the genus Chrysosporium, the genus Saccharomyces, the genus Stenotrophamonas, the genus Schizosaccharomyces, the genus Yarrowia, the genus Streptomyces, a Bacillus lentus cell, a Bacillus brevis cell, a Bacillus stearothermophilus cell, a Bacillus licheniformis cell, a Bacillus alkalophilus cell, a Bacillus coagulans cell, a Bacillus circulans cell, a Bacillus pumilis cell, a Bacillus thuringiensis cell, a Bacillus clausii cell, a Bacillus megaterium cell, a Bacillus subtilis cell, a Bacillus amyloliquefaciens cell, a Trichoderma koningii cell, a Trichoderma viride cell, a Trichoderma reesei cell, a Trichoderma longibrachiatum cell, an Aspergillus awamori cell, an Aspergilus fumigates cell, an Aspergillus foetidus cell, an Aspergillus nidulans cell, an Aspergillus niger cell, an Aspergillus oryzae cell, a Humicola insolens cell, a Humicola lanuginose cell, a Rhodococcus opacus cell, a Rhizomucor miehei cell, a Mucur michei cell, a Streptomyces lividans cell, a Streptomyces murinus cell, an Actinomycetes cell, a CHO cell, a COS cell, a VERO cell, a BHK cell, a HeLa cell, a Cv1 cell, an MDCK cell, a 293 cell, a 3T3 cell, a PC12 cell, an E. coli cell, a strain B E. coli cell, a strain C E. coli cell, a strain K E. coli cell, and a strain W E. coli cell.

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12 . The method of claim 1 , wherein the fatty ester production host produces fatty acid methyl esters at a titer of about 100 mg/L or more.

13 . The method of claim 1 , wherein the fatty ester production host has a specific productivity for fatty ester of about 30 mg/L/OD 600 or more.

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28 . A fatty ester composition, comprising:

a production host;

a fatty acid methyl ester having the following formula:

BCOOCH 3

wherein B is a carbon chain that is at least 6 carbons in length.

29 . The fatty ester composition of claim 28 , wherein the fatty ester production host comprises a heterologous nucleic acid sequence encoding an ester synthase.

30 . The fatty ester composition of claim 28 , wherein the fatty ester production host comprises a heterologous nucleic acid sequence encoding a thioesterase.

31 . The fatty ester composition of claim 28 , wherein the fatty ester production host comprises a heterologous nucleic acid sequence encoding an acyl-CoA synthase.

32 . (canceled)

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38 . The fatty ester composition of claim 28 , wherein the fatty acid methyl ester has a fraction of modern carbon of about 1.003 to about 1.5.

39 . The fatty ester composition of claim 28 , wherein the fatty ester has a δ 13 C of from about −10.9 to about −15.4.

40 . A biofuel comprising the fatty ester compositions of claim 38 .

41 . A biofuel comprising the fatty ester composition of claim 39 .

42 . (canceled)

43 . A fatty acid methyl ester produced by the method of claim 1 .