Genetic loci associated with head smut resistance in maize
Head smut is one of the most devastating diseases in maize, causing severe yield loss worldwide. The present invention describes the fine-mapping of a major QTL conferring resistance to head smut. Markers useful for breeding, and methods for conferring head smut resistance are described. Nucleic acid sequence from the genetic locus conferring head smut resistance is disclosed. Genes encoding proteins conferring head smut resistance are disclosed.
1. A method of identifying a maize plant that displays head smut resistance, the method comprising:
A. isolating nucleic acid molecules from a maize plant;
B. analyzing the isolated nucleic acid molecules for the presence of a QTL allele that is associated with head smut resistance, wherein the presence of said QTL allele is determined by detecting in the germplasm of the maize plant at least one allele of a marker locus wherein:
(a) the marker locus is located within a chromosomal interval comprising and flanked by umc1736 and umc2184; and
(b) the at least one allele is associated with head smut resistance; and
C. selecting said maize plant if said QTL allele is detected, wherein the selected plant displays head smut resistance.
2. The method of claim 1 , wherein the marker locus is located within a chromosomal interval comprising and flanked by SSR148152 and SNP661.
3. A method of marker assisted selection of a maize plant that displays head smut resistance comprising:
(a) obtaining a first maize plant having at least one marker allele selected from the group consisting of:
(i) the sequence set forth in SEQ ID NO:23;
(ii) the sequence set forth in SEQ ID NO:54;
(iii) the sequence set forth in SEQ ID NO:58;
(iv) the sequence set forth in SEQ ID NO:61;
(v) the sequence set forth in SEQ ID NO:64;
(vi) the sequence set forth in SEQ ID NO:67;
(vii) the sequence set forth in SEQ ID NO:70; and
(viii) the sequence set forth in SEQ ID NO:24;
(b) crossing said first maize plant to a second maize plant;
(c) detecting the presence of the marker allele in the progeny of the crossing; and
(d) selecting a progeny maize plant that possesses the at least one marker allele detected, wherein the selected progeny maize plant displays head smut resistance.
4. A method of detecting a head smut resistance locus in a maize plant comprising:
A. isolating nucleic acid molecules from a maize plant;
B. analyzing the isolated nucleic acid molecules for the presence of a QTL allele that is associated with head smut resistance, wherein the presence of said QTL allele is determined by detecting the presence in the maize plant of:
a) the sequence set forth in SEQ ID NO:23,
b) the sequence set forth in SEQ ID NO:54,
c) the sequence set forth in SEQ ID NO:58,
d) the sequence set forth in SEQ ID NO:61,
e) the sequence set forth in SEQ ID NO:64,
f) the sequence set forth in SEQ ID NO:67,
g) the sequence set forth in SEQ ID NO:70, or
h) the sequence set forth in SEQ ID NO:24; and
C. selecting said maize plant if said QTL allele is detected, wherein the selected maize plant displays head smut resistance.