Methods and compositions for the production of monoclonal antibodies
View Patent ↗The present invention comprises compositions and methods for making monoclonal antibodies. The present invention further comprises vectors that replicate the immune system components, particularly an antigen-presenting cell (APC) element of the immune synapse. Additionally, the present invention may further comprise synthetic T-cells.
1. A composition, comprising a synthetic antigen presenting cell (APC), wherein the synthetic APC comprises a major histocompatibility complex (MHC) protein bound to a first colloidal metal particle, a structural protein bound to a second colloidal metal particle, and a co-stimulatory protein B7 bound to a third colloidal metal particle, wherein the first, second and third colloidal metal particles are bound to each other by scaffolding molecules.
2. The composition of claim 1 , wherein the MHC protein, the structural protein and the co-stimulatory protein B7 are bound indirectly by a binding pair.
3. The composition of claim 2 , wherein the binding pair comprises streptavidin-biotin.
4. The composition of claim 1 , wherein the structural protein is selected from the group consisting of: intracellular adhesion molecule (ICAM), LFA-3, and CD72.
5. The composition of claim 1 , wherein the colloidal metal comprises colloidal gold, colloidal silver, colloidal iron, colloidal aluminum, or colloidal platinum.
6. The composition of claim 1 , further comprising a pharmaceutically-acceptable component comprising excipients, buffers or carriers.
7. The composition of claim 1 , further comprising an adjuvant, wherein the adjuvant comprises liposomes, emulsions, microspheres, biodegradable polymers and polystyrene, alum, heat killed M. butyricum and M. tuberculosis , Pertussis toxin and Tetanus toxin, or LPS and Staphylococcal enterotoxin B.
8. The composition of claim 1 , wherein the MHC protein is an antigen-loaded MHC protein.
9. The composition of claim 1 wherein the colloidal metal particles are the same size.
10. The composition of claim 1 , wherein the colloidal metal particles are different sizes.
11. The composition of claim 10 , wherein the first colloidal metal particle is 32 nanometers (nm), and the second and third colloidal metal particles are 17 nm.
12. The composition of claim 1 , wherein the first, second and third colloidal metal particles are coated with streptavidin and wherein the scaffolding molecule is a biotinylated protein.
13. The composition of claim 12 , wherein the biotinylated protein is human serum albumin (HSA).
14. The composition of claim 1 , wherein the scaffolding molecule is a di-thiol alkane.
15. The composition of claim 1 , wherein the scaffolding molecule is a 2 or 4-arm poly-ethylene glycol (PEG).