Methods for prenatal diagnosis of chromosomal abnormalities
View Patent ↗Chromosomal abnormalities are responsible for a significant number of birth defects, including mental retardation. The present invention is related to methods for non-invasive and rapid, prenatal diagnosis of chromosomal abnormalities based on analysis of a maternal blood sample. The invention exploits the differences in DNA between the mother and fetus, for instance differences in their methylation states, as a means to enrich for fetal DNA in maternal plasma sample. The methods described herein can be used to detect chromosomal DNA deletions and duplications. In a preferred embodiment, the methods are used to diagnose chromosomal aneuploidy and related disorders, such as Down's and Turner's Syndrome.
1. A method for enriching feta DNA in a maternal plasma sample, comprising digesting the DNA present in said sample with a methyl-sensitive enzyme that selectively and substantially completely digests maternal DNA to obtain a sample enriched for fetal nucleic acids.
2. The method of claim 1 , further comprising a nucleic acid amplification step, wherein after digestion fetal nucleic acid is amplified preferentially.
3. The method of claim 1 , wherein said methyl-sensitive enzyme digests only at nucleic acid recognition sites that are unmethylated.
4. The method of claim 1 , wherein said methyl-sensitive enzyme digests only at nucleic acid recognition sites that are methylated.
5. The method of claim 1 , wherein the DNA is isolated from the plasma sample before it is digested with a methyl-sensitive enzyme.
6. The method of claim 1 or 5 , further comprising a step of isolating the undigested fetal DNA after digesting the maternal DNA in said sample with a methyl-sensitive enzyme.
7. The method of claim 1 , wherein the step of digesting the DNA present in said sample is performed using two or more methyl-sensitive enzymes.