Pressure Chamber Clamp Mechanism
In a high-density sequence detection system, an apparatus for transporting a microplate. A control system provides a thermocycler control signal to regulate a desired thermal output of a thermocycler block. A frame has a first side, a second side, and an opening that extends through the first side and the second side. A window is positioned in the opening and a circumferential seal is positioned around a periphery of the opening at the second side. The seal has a peripheral lip that seals against the microplate. A clamp is adapted to engage and displace the frame from an unclamped position to a clamped position, wherein in the unclamped position, the microplate is displaced away from the thermocycler block and in the clamped position, the clamp urges the microplate against the thermocycler block. In some embodiments the peripheral lip is positioned radially inward of the periphery of the opening.
1 . An thermocycling apparatus comprising:
a thermocycler block adapted to accept a microplate, said microplate having a first surface and an opposing second surface and a plurality of wells formed in the first surface;
a first sealing member having a top surface and an opposing bottom surface, wherein the opposing bottom surface is adjacent to the first surface of the microplate, thereby forming a plurality of sealed wells and a sealed microplate; and
a pressure chamber filled with a fluid pressurized to exert a force upon the top surface of the first sealing member, thereby sealing the microplate during thermocycling;
wherein the first sealing member and the pressure chamber allow transmission of electromagnetic radiation.
2 . The apparatus of claim 1 , wherein said pressure chamber comprises:
a circumferential chamber seal engaging at least one of the thermocycler and the top surface of the first sealing member.
3 . The apparatus of claim 1 , wherein said first sealing member comprises an adhesive layer disposed on the bottom surface.
4 . The apparatus of claim 1 , wherein the pressure of said pressurized gas is about 18 PSI while a temperature of said microplate is greater than 40 degrees Celsius.
5 . A method of maintaining a sealed microplate during thermocycling, said method comprising:
providing a thermocycling apparatus comprising a pressure chamber filled with a fluid pressurized to exert a force upon a top surface of a microplate during thermocycling;
placing a sealed microplate having a plurality of wells on a thermocycler block, wherein the sealed microplate has been prepared for performing PCR;
positioning the pressure chamber over the sealed microplate; and
thermocycling the sealed microplate to permit PCR to occur, wherein the pressure chamber seals the microplate during thermocycling, and wherein the pressure chamber allows transmission of electromagnetic radiation.
6 . The method of claim 5 , wherein the pressure of said pressurized gas is about 18 PSI while a temperature of said microplate is greater than 40 degrees Celsius.