Ligand graft functionalized substrates
View Patent ↗Polyethyleneimine and polyalkylene biguanide ligand functionalized substrates, methods of making ligand functionalized substrates, and methods of using functionalized substrates are disclosed.
1. An article comprising a substrate and extending from the surfaces thereof:
grafted polyethyleneimine ligand groups forming a ligand-functionalized substrate;
wherein the polyethyleneimine ligand groups are one or more of the formula:
wherein:
“˜” is a divalent linking group grafted to the surface of the substrate and the polyethyleneimine ligand group,
x may be zero and y is at least 1, and x+y is 2 to 50; and
grafted quaternary ammonium groups on the surface of the substrate.
2. An article comprising a porous base substrate having interstitial and outer surfaces, grafted quaternary ammonium groups, and grafted ligand groups extending from the surfaces of the porous base substrate thereby forming a ligand-functionalized substrate; wherein the grafted ligand groups comprises the reaction product of a grafted electrophilic functional group and a ligand compound:
wherein x is zero, y is at least 1, and x+y is 2 to 2000.
3. An article comprising a substrate and extending from the surfaces thereof grafted polyethyleneimine ligand groups and grafted quaternary ammonium groups, thereby forming a ligand-functionalized substrate;
wherein the polyethyleneimine ligand groups are one or more of the formula:
wherein:
“˜” is a divalent linking group grafted to the surface of the substrate and the polyethyleneimine ligand group,
x may be zero and y is at least 1, and x+y is 2 to 50;
wherein the ligand-functionalized substrate has enhanced binding selectivity for host cell proteins, DNA, RNA, and viruses relative to antibodies; and
wherein the article yields a log-reduction value (LRV) of at least 5.0 for neutral viruses disposed in a solution comprising from 0 to about 150 mM salt.