Gibberellin 2-oxidase
View Patent ↗A nucleic acid sequence is provided which encodes a gibberellin 2-oxidase gene which catalyses the 2β-oxidation of a gibberellin molecule to introduce a hydroxyl group at C-2 and further catalyses the oxidation of the hydroxyl group introduced at C-2 to yield the ketone derivative. Such sequences can find application in the preparation of transgenic plants with altered levels of gibberellin 2-oxidase.
1. An isolated antisense nucleic acid complementary to all or part of SEQ ID NO:1 wherein said antisense nucleic acid hybridizes to SEQ ID NO:1 under stringent conditions that comprise hybridization at 65° C. and washing in 0.1×SSC/0.1% SDS at 68° C., and wherein expression of said antisense nucleic acid in a P. coccineus plant cell reduces expression of the endogenous P. coccineus gibberellin 2-oxidase gene.
2. A vector comprising the antisense nucleic acid of claim 1 .
3. An isolated host cell transfected or transformed with the antisense nucleic acid of claim 1 .
4. A host cell transfected or transformed with the antisense nucleic acid of claim 1 wherein said host cell is a plant cell.
5. A plant or a part of a plant that comprises the host cell of claim 4 .
6. Propagating material from the plant of claim 5 , wherein said propagation material comprises the antisense nucleic acid that was introduced into said plant.
7. A method for preparing a plant comprising introducing said antisense nucleic acid of claim 1 into a plant cell and regenerating a plant from said plant cell.
8. A method for reducing expression of endogenous gibberellin 2-oxidase in a transgenic plant, comprising
(a) preparing a plant that comprises the antisense nucleic acid of claim 1 ; and
(b) expressing said antisense nucleic acid
wherein expression of said antisense nucleic acid reduces expression of endogenous gibberellin 2-oxidase enzyme in said transgenic plant.
9. The isolated antisense nucleic acid of claim 1 , wherein said nucleic acid is DNA.
10. The isolated antisense nucleic acid of claim 1 , wherein said nucleic acid is RNA.
11. A construct comprising the isolated antisense nucleic acid of claim 1 operably linked to a promoter that is functional in a plant cell.
12. A plant cell comprising the construct of claim 11 .
13. A method of promoting plant growth, comprising:
(a) transforming a plant cell with the construct of claim 11 ;
(b) regenerating a plant from said transformed plant cell; and
(c) expressing said antisense nucleic acid.
14. The isolated antisense nucleic acid of claim 1 , wherein said antisense nucleic acid has at least 80% nucleic acid sequence identity to the complementary strand of residues 68-1063 of SEQ ID No. 1.