Fiber spectroscopic probe mountable on a microscope
View Patent ↗A fiber spectroscopic probe that can be mounted directly above the objective lens of a standard microscope to add a spectroscopic function to the microscope. The fiber spectroscopic probe only consists of a minimum number of optical components and is compact enough to induce minimum alteration to the optical path of the microscope.
1. A fiber spectroscopic probe mountable on a standard light microscope, said fiber spectroscopic probe comprising:
an input optical fiber for delivering excitation light from an external light source;
an adapter means mountable directly above an objective lens of the microscope for directing said excitation light through said objective lens to a sample to excite a spectroscopic signal and collecting said spectroscopic signal though said objective lens; and
an output optical fiber for delivering said spectroscopic signal to an external spectrometer device for spectral analysis.
2. The fiber spectroscopic probe of claim 1 , wherein said adapter means does not occupy any viewing port of the microscope.
3. The fiber spectroscopic probe of claim 1 , wherein said spectroscopic signal is a Raman scattering signal.
4. The fiber spectroscopic probe of claim 1 , wherein said spectroscopic signal is a fluorescence signal.
5. The fiber spectroscopic probe of claim 1 , wherein said adapter means comprises a beam-splitting member.
6. The fiber spectroscopic probe of claim 5 , wherein said beam-splitting member has a wavelength-dependent transmissive/reflective property.
7. The fiber spectroscopic probe of claim 1 , further comprising a spatial pinhole before said output optical fiber.