Genetic loci associated with northern leaf blight resistance in maize
The invention relates to methods and compositions for identifying and selecting maize plants with enhanced resistance to Exserohilum and/or northern leaf blight. Maize plants generated by the methods of the invention are also a feature of the invention.
1. A method of identifying a maize plant that displays resistance to Exserohilum and/or northern leaf blight, the method comprising:
A. isolating nucleic acids from a maize plant;
B. analyzing the isolated nucleic acids for the presence of a haplotype associated with resistance to Exserohilum and/or northern leaf blight, wherein said haplotype is located within a chromosomal interval comprising and flanked by PHM1784 and PHM2381 and comprises:
i. a “G” at PHM4743.50; a “G” at PHM18903.29; a “C” at PHM8340.5; and a “C” at PHM9343.12; or
ii. an “A” at PHM4743.50; an “A” at PHM18903.29; a “T” at PHM8340.5; and a “T” at PHM9343.12; and
C. selecting the maize plant if haplotype (i) or haplotype (ii) is detected.
2. A method of marker assisted selection comprising:
A. obtaining a first maize plant that displays resistance to Exserohilum and/or northern leaf blight, wherein said maize plant has a haplotype within a chromosomal interval comprising and flanked by PHM1784 and PHM2381 said haplotype comprising:
i. a “G” at PHM4743.50; a “G” at PHM18903.29; a “C” at PHM8340.5; and a “C” at PHM9343.12; or
ii. an “A” at PHM4743.50; an “A” at PHM18903.29; a “T” at PHM8340.5; and a “T” at PHM9343.12;
B. crossing said first maize plant to a second maize plant;
C. evaluating the progeny for haplotype (i) or haplotype (ii); and
D. selecting progeny plants that possess haplotype (i) or haplotype (ii) of the first maize plant.