Method of increasing red blood cell levels or treating anemia in a patient
In certain aspects, the present invention provides compositions and methods for increasing red blood cell and/or hemoglobin levels in vertebrates, including rodents and primates, and particularly in humans.
1. A method for increasing red blood cell levels or treating anemia in a patient, the method comprising administering to a patient in need thereof an effective amount of a polypeptide comprising an amino acid sequence that is at least 95% identical to the sequence of amino acids 29-109 of SEQ ID NO: 1, and wherein the polypeptide comprises an acidic amino acid at the position corresponding to position 79 of SEQ ID NO:1.
2. The method of claim 1 , wherein the acidic amino acid is glutamic acid.
3. The method of claim 1 , wherein the polypeptide comprises an amino acid sequence that is at least 95% identical to the sequence of amino acids 25-118 of SEQ ID NO: 1.
4. The method of claim 1 , wherein the polypeptide comprises an amino acid sequence that is at least 95% identical to the sequence of amino acids 25-128 of SEQ ID NO: 1.
5. The method of claim 1 , wherein the polypeptide comprises an amino acid sequence that is at least 97% identical to the sequence of amino acids 25-131 of SEQ ID NO: 1.
6. The method of claim 1 , wherein the polypeptide comprises the amino acid sequence of SEQ ID NO: 7.
7. The method of claim 1 , wherein the patient has anemia associated with a kidney disorder.
8. The method of claim 7 , wherein the patient has anemia associated with chronic kidney disease.
9. The method of claim 8 , wherein the polypeptide has an amino acid sequence consisting of SEQ ID NO:28.
10. The method of claim 7 , wherein the polypeptide has an amino acid sequence consisting of SEQ ID NO:28.
11. The method of claim 1 , wherein the patient has anemia associated with a chemotherapy treatment.
12. The method of claim 11 , wherein the chemotherapy treatment is a taxane.
13. The method of claim 12 , wherein the polypeptide has an amino acid sequence consisting of SEQ ID NO:28.
14. The method of claim 11 , wherein the polypeptide has an amino acid sequence consisting of SEQ ID NO:28.
15. The method of claim 1 , wherein the patient has anemia as a consequence of blood loss.
16. The method of claim 15 , wherein the polypeptide has an amino acid sequence consisting of SEQ ID NO:28.
17. The method of claim 1 , wherein the acidic amino acid is aspartic acid.
18. The method of claim 1 , wherein the polypeptide comprises the amino acid sequence of SEQ ID NO:28.
19. The method of claim 1 , wherein the polypeptide comprises the amino acid sequence of SEQ ID NO:29.
20. The method of claim 1 , wherein the polypeptide comprises the amino acid sequence of SEQ ID NO:32.
21. The method of claim 1 , wherein the polypeptide comprises the amino acid sequence of SEQ ID NO:37.
22. The method of claim 1 , wherein the polypeptide comprises the amino acid sequence of SEQ ID NO:38.
23. The method of claim 1 , wherein the polypeptide has an amino acid sequence consisting of SEQ ID NO:7.
24. The method of claim 1 , wherein the polypeptide has an amino acid sequence consisting of SEQ ID NO:28.
25. The method of claim 1 , wherein the polypeptide has an amino acid sequence consisting of SEQ ID NO:29.
26. The method of claim 1 , wherein the polypeptide has an amino acid sequence consisting of SEQ ID NO:32.
27. The method of claim 1 , wherein the polypeptide has an amino acid sequence consisting of SEQ ID NO:37.
28. The method of claim 1 , wherein the polypeptide has an amino acid sequence consisting of SEQ ID NO:38.