SiRNA targeting ras-related nuclear protein RAN
View Patent ↗Efficient sequence specific gene silencing is possible through the use of siRNA technology. By selecting particular siRNAs by rational design, one can maximize the generation of an effective gene silencing reagent, as well as methods for silencing genes. Methods, compositions, and kits generated through rational design of siRNAs are disclosed.
1. An siRNA molecule, wherein said siRNA molecule consists of: (a) a duplex region; and (b) either no overhang regions or at least one overhang region, wherein each overhang region contains six or fewer nucleotides, wherein the duplex region consists of a sense region and an antisense region, wherein said sense region and said antisense region together form said duplex region and said duplex region is 19-30 base pairs in length and said antisense region comprises a sequence that is the complement of SEQ ID NO: 458 or SEQ ID NO: 453.
2. The siRNA molecule of claim 1 , wherein said antisense region and said sense region are each 19-25 nucleotides in length.
3. The siRNA molecule of claim 2 , wherein said antisense region and said sense region are each 19 nucleotides in length.
4. The siRNA molecule of claim 1 , wherein said siRNA molecule has at least one overhang region.
5. The siRNA molecule of claim 1 , wherein said siRNA molecule has no overhang regions.
6. The siRNA molecule of claim 2 , wherein said siRNA molecule has at least one overhang region.
7. The siRNA molecule of claim 2 , wherein said siRNA molecule has no overhang regions.
8. The siRNA molecule of claim 3 , wherein said siRNA molecule has at least one overhang region.
9. The siRNA molecule of claim 3 , wherein said siRNA molecule has no overhang regions.
10. The siRNA molecule of claim 1 , wherein said siRNA molecule comprises a sequence that is the complement of SEQ ID NO: 458.
11. The siRNA molecule of claim 1 , wherein said siRNA molecule comprises a sequence that is the complement of SEQ ID NO: 453.
12. A chemically synthesized double stranded siRNA molecule, wherein:
(a) each strand of said siRNA molecule is between 19 and 30 nucleotides in length; and
(b) one strand of said siRNA molecule comprises a sequence that is the complement of SEQ ID NO: 458 or SEQ ID NO: 453.
13. The siRNA molecule of claim 12 , wherein each strand of said siRNA molecule is 19-25 nucleotides in length.
14. The siRNA molecule of claim 13 , wherein each strand of said siRNA molecule is 19 nucleotides in length.
15. The siRNA molecule of claim 12 , wherein said one strand of said siRNA molecule comprises a sequence that is the complement of SEQ ID NO: 458.
16. The siRNA molecule of claim 12 , wherein said one strand of said siRNA molecule comprises a sequence that is the complement of SEQ ID NO: 453.
17. A pool of at least two siRNAs, wherein said pool comprises a first siRNA and a second siRNA, wherein
(a) said first siRNA consists of the siRNA molecule of claim 10 , wherein the duplex region of said first siRNA is a first duplex region; and
(b) said second siRNA consists of: (i) a second duplex region; and (ii) either no overhang regions or at least one overhang region that contains six or fewer nucleotides, wherein the second duplex region consists of a sense region and an antisense region, wherein said sense region and said antisense region of said second siRNA together form said second duplex region and said second duplex region is 19-30 base pairs in length and said antisense region of said second siRNA comprises a sequence that is the complement of a sequence selected from the group consisting of SEQ ID NO: 438-448, 450-457 and 459-465.
18. The pool of claim 17 , wherein said first siRNA and said second siRNA each comprise no overhang regions.
19. The pool of claim 17 , wherein said first duplex region and said second duplex region are each 19-25 base pairs in length.
20. The pool of claim 19 , wherein said first duplex region and said second duplex region are each 19 base pairs in length.
21. A pool of at least two siRNAs, wherein said pool comprises a first siRNA and a second siRNA, wherein
(a) said first siRNA consists of the siRNA molecule of claim 11 , wherein the duplex region of said first siRNA is a first duplex region; and
(b) said second siRNA consists of: (i) a second duplex region; and (ii) either no overhang regions or at least one overhang region that contains six or fewer nucleotides, wherein the second duplex region consists of a sense region and an antisense region, wherein said sense region and said antisense region of said second siRNA together form said second duplex region and said second duplex region is 19-30 base pairs in length and said antisense region of said second siRNA comprises a sequence that is the complement of a sequence selected from the group consisting of SEQ ID NO: 438-452 and 454-465.
22. The pool of claim 21 , wherein said first siRNA and said second siRNA each comprise no overhang regions.
23. The pool of claim 21 , wherein said first duplex region and said second duplex region are each 19-25 base pairs in length.
24. The pool of claim 23 , wherein said first duplex region and said second duplex region are each 19 base pairs in length.