Methods for quantifying protein leakage from protein based affinity chromatography resins
View Patent ↗The present invention provides methods of quantifying protein leakage from a protein based affinity chromatography media (e.g., protein A, protein G and protein L based affinity chromatography media), where such a protein is used for isolating and/or removing a molecule which binds the protein (e.g., an immunoglobulin).
1. A method for quantifying PrA leakage from a PrA based affinity chromatography resin, the method comprising the steps of:
(a) labeling the PrA in the PrA based chromatography resin with one or more fluorescent tags;
(b) dividing the labeled resin into equal first and second parts;
(c) treating first part with suitable means to release the labeled PrA from the resin and adding an excess amount of immunoglobulin (Ig) to the second part;
(d) measuring fluorescence (FL digest ) and PrA concentration ([PrA]) from the treated resin in the first part in step (c); and
(e) measuring resin Ig eluate concentration ([Ig]) and fluorescence signal in an Ig eluate (FL Ig ) from the second part in step (c),
wherein PrA leakage is quantified by calculating {(FL Ig )/((FL digest )/[PrA]))}[Ig].
2. The method of claim 1 , wherein the immunoglobulin is an IgG.
3. The method of claim 1 , further comprising the step of removing the leached PrA using one or more of cation exchange chromatography, anion exchange chromatography and hydrophobic exchange chromatography, weak partitioning chromatography, hydroxyapatite chromatography, or any combinations thereof.
4. The method of claim 1 , wherein the fluorescent tag is an amine reactive dye.
5. The method of claim 1 , wherein the suitable means comprises chemical treatment.
6. The method of claim 1 , wherein the suitable means comprises enzymatic treatment.
7. The method of claim 6 , wherein the enzymatic treatment comprises the use of trypsin, pepsin, chymotrypsin, thermolysin and subtlelysin.
8. The method of claim 5 , wherein the chemical treatment comprises use of an acid or base.
9. The method of claim 1 , wherein the fluorescence is measured using a fluorometer.
10. The method of claim 1 , wherein the Ig is eluted using a suitable pH.
11. The method of claim 1 , wherein the Ig is monoclonal.
12. The method of claim 1 , wherein the Ig is polyclonal.
13. The method of claim 1 , wherein the immunoglobulin is an IgG.