Emulsion compositions
View Patent ↗An emulsion is useful in allowing a wide variety of gene products to be expressed via eukaryotic in vitro expression. The emulsion comprises a silicone based surfactant, a hydrophobic phase and a hydrophilic phase; wherein the hydrophilic phase comprises a plurality of compartments containing a functional in vitro eukaryotic expression system.
1. An emulsion comprising a surfactant, a hydrophobic phase and a hydrophilic phase comprising a plurality of microcapsules containing a system for increasing the effective concentration of a nucleic acid molecule, wherein the surfactant is a chemically inert silicone-based surfactant and wherein the microcapsules have a sphere of diameter between 0.1 μm and 10 μm.
2. The emulsion of claim 1 wherein the emulsion comprises a hydrophilic phase to hydrophobic phase ratio of 1 to 2.5.
3. The emulsion of claim 1 wherein the nucleic acid molecule is genomic DNA or cDNA.
4. The emulsion of claim 1 wherein the nucleic acid molecule comprises a tag selected from the group consisting of biotin, digoxigenin, and 2,4-dinitrophenyl,
5. The emulsion of claim 1 , wherein the chemically inert silicone-based surfactant is a silicone copolymer.
6. The emulsion of claim 1 , wherein the silicone copolymer is polysiloxane-polycetyl-polyethylene glycol copolymer.
7. The emulsion of claim 1 , wherein the chemically inert silicone-based surfactant is Abil EM90.
8. The emulsion of claim 1 , wherein the emulsion is thermostable.
9. The emulsion of claim 1 wherein the chemically inert silicone-based surfactant is provided at a v/v concentration range of 0.5-20% of surfactant in oil.
10. The emulsion of claim 1 wherein the plurality of microcapsules when formed each contains on average one or less than one nucleic acid molecule.
11. The emulsion of claim 1 wherein the plurality of microcapsules when formed each contains on average between 5 and 1000 nucleic acid molecules.