Method of producing an inseminate
View Patent ↗A reduced dose inseminate and method for producing and using such reduced dose inseminate to fertilize the eggs of a female animal by artificial insemination with fewer sperm cells than compared with conventional dose inseminates.
1. A method of producing an inseminate comprising:
a) obtaining a plurality of sex-selected bovine sperm cells;
b) obtaining a plurality of heterogeneous sperm cells, wherein the heterogeneous sperm cells comprise either a plurality of sperm cells obtained from a species other than bovine, or a plurality of infertile bovine sperm cells;
c) combining said plurality of heterogeneous sperm cells with said plurality of sex-selected bovine sperm cells;
d) providing within a combination of said sex-selected bovine sperm cells and said heterogeneous sperm cells a plurality of sex-selected bovine sperm cells sufficient to achieve fertilization of a bovine egg.
2. The method of claim 1 , further comprising the step of fertilizing said bovine egg in vivo with said combination of sex-selected bovine sperm cells and heterogeneous sperm cells.
3. The method of claim 2 , further comprising the step of artificially inseminating a female bovine with said combination of sex-selected bovine sperm cells and heterogeneous sperm cells.
4. The method of claim 3 , further comprising the step of achieving a pregnancy of said female bovine by artificially inseminating said female bovine with said combination of sex-selected bovine sperm cells and heterogeneous sperm cells.
5. The method of claim 4 , further comprising the step of producing a bovine offspring from said pregnancy of said female bovine.
6. The method of claim 1 , further comprising the step of providing in said combination fewer of said plurality of sex-selected bovine sperm cells as compared to said plurality of heterogeneous sperm cells.
7. The method of claim 6 , further comprising the step of allocating to said combination said plurality of sex-selected bovine sperm cells in a range of about five percent to about fifty percent of said plurality of heterogeneous sperm cells.
8. The method of claim 1 , wherein said plurality of heterogeneous sperm cells comprise a plurality of sperm cells obtained from a species other than bovine and are incapable of fertilizing said bovine egg.
9. The method of claim 1 , wherein said plurality of heterogeneous sperm cells comprise infertile bovine sperm cells incapable of fertilization of said bovine egg.
10. The method of claim 1 , further comprising the step of mixing said plurality of sex-selected bovine sperm cells with an amount of TRIS A extender to achieve a concentration of said plurality of sex-selected bovine sperm cells in a range of about 8 million per mL to about 16 million per mL.
11. The method of claim 10 , further comprising the step of mixing with said plurality of heterogeneous sperm cells an amount of TRIS A extender to achieve a concentration of said plurality of heterogeneous sperm cells in a range of about 32 million per mL to about 48 million per mL.
12. The method of claim 1 , wherein said step of combining said plurality of heterogeneous sperm cells with said plurality of sex-selected bovine sperm cells results in a concentration of sex-selected bovine sperm cells in a range of about 4 million sperm cells per mL and about 8 million sperm cells and a concentration of said heterogeneous sperm cells in a range of about 16 million sperm cells per mL and about 24 million sperm cells per mL.
13. The method of claim 12 , further comprising the step of adding an amount of TRIS B to achieve a concentration of sex-selected bovine sperm cells in a range of about 2 million sperm cells per mL and about 4 million sperm cells and a concentration of heterogeneous sperm cells in a range of about 8 million sperm cells per mL and about 12 million sperm cells per mL.
14. The method of claim 13 , further comprising the step of providing an artificial insemination dosage comprising an artificial insemination straw having a volume in a range of about 0.25 mL and about 0.5 mL which contains sex-selected bovine sperm cells in a range of about 0.5 million to 2 million and heterogeneous sperm cells in a range of 2 million to about 6 million.
15. The method of claim 14 , further comprising the step of freezing said artificial insemination dosage.
16. The method of claim 14 , further comprising the step of thawing said artificial insemination dosage.
17. The method of claim 14 , wherein said TRIS A comprises TRIS A2, and wherein said TRIS B comprises TRIS B2.