Methods and materials relating to stem cell growth factor-like polypeptides and polynucleotides
The invention provides novel polynucleotides and polypeptides encoded by such polynucleotides and mutants or variants thereof that correspond to a novel human secreted stem cell growth factor-like polypeptides. Other aspects of the invention include vectors containing processes for producing novel human secreted stem cell growth factor-like polypeptides, and antibodies specific for such polypeptides.
1 . An isolated polynucleotide comprising a coding sequence encoding a polypeptide comprising an amino acid sequence with at least 95% sequence identity to the amino acid sequence of SEQ ID NO:18 or 27.
2 . The polynucleotide of claim 1 , wherein said polynucleotide comprises a coding sequence encoding a polypeptide comprising an amino acid sequence with at least 99% sequence identity to the amino acid sequence of SEQ ID NO:18 or 27.
3 . The polynucleotide of claim 1 , wherein said polynucleotide comprises a coding sequence encoding a polypeptide comprising the amino acid sequence of SEQ ID NO: 18 or 27.
4 . The polynucleotide of claim 1 , wherein said polynucleotide comprises the nucleotide sequence of SEQ ID NO:17 or 26, or the mature protein coding portion thereof.
5 . A recombinant vector comprising:
(a) the polynucleotide of claim 1 ; and
(b) at least one control element operably linked to said polynucleotide, whereby said coding sequence can be transcribed and translated in a host cell.
6 . A recombinant vector comprising:
(a) the polynucleotide of claim 2 ; and
(b) at least one control element operably linked to said polynucleotide, whereby said coding sequence can be transcribed and translated in a host cell.
7 . A recombinant vector comprising:
(a) the polynucleotide of claims 3 ; and
(b) at least one control element operably linked to said polynucleotide, whereby said coding sequence can be transcribed and translated in a host cell.
8 . An isolated host cell comprising the recombinant vector of claim 5 .
9 . An isolated host cell comprising the recombinant vector of claim 6 .
10 . An isolated host cell comprising the recombinant vector of claim 7 .
11 . The host cell of claim 8 , wherein the cell is a mammalian cell.
12 . The host cell of claim 11 , wherein the mammalian cell is a CHO cell.
13 . A method for producing a recombinant polypeptide, said method comprising culturing a population of host cells according to claim 8 under conditions for producing said polypeptide.
14 . A method for producing a recombinant polypeptide, said method comprising culturing a population of host cells according to claim 9 under conditions for producing said polypeptide.
15 . A method for producing a recombinant polypeptide, said method comprising culturing a population of host cells according to claim 10 under conditions for producing said polypeptide.
16 . A method for producing a recombinant polypeptide, said method comprising culturing a population of host cells according to claim 11 under conditions for producing said polypeptide.
17 . A method for producing a recombinant polypeptide, said method comprising culturing a population of host cells according to claim 12 under conditions for producing said polypeptide.
18 . A nucleic acid array comprising the polynucleotide of claim 1 attached to a surface.
19 . A nucleic acid array comprising the polynucleotide of claim 2 attached to a surface.
20 . A nucleic acid array comprising the polynucleotide of claim 3 attached to a surface.
21 . A polynucleotide which is the complement of the polynucleotide of claim 1 .
22 . A polynucleotide which is the complement of the polynucleotide of claim 2 .
23 . A polynucleotide which is the complement of the polynucleotide of claim 3 .