Event EE-GH5 insect resistant cotton plants and methods of using
View Patent ↗The invention provides specific transgenic cotton plants, plant material and seeds, characterized in that these products harbor a specific transformation event at a specific location in the cotton genome. Tools are also provided which allow rapid and unequivocal identification of the event in biological samples.
1. A transgenic cotton plant, cells, parts, seed, or progeny thereof, each comprising elite event EE-GH5 in its genome, reference seed comprising said event having being deposited at the ATCC under deposit number PTA-8171.
2. The transgenic cotton plant, seed, cells, parts, or progeny of claim 1 , wherein the genomic DNA of which, when analyzed using the Elite event identification protocol for EE-GH5 with two primers comprising the nucleotide sequence of SEQ ID No 10 and SEQ ID No 11 respectively, yields a DNA fragment of about 262 bp or when analyzed using the Elite event identification protocol for EE-GH5 with two primers comprising the nucleotide sequence of SEQ ID No 3 and SEQ ID No 6 respectively, yields a DNA fragment of about 369 bp.
3. A seed comprising elite event EE-GH5 deposited at the ATCC under deposit number PTA-8171 or derivatives therefrom.
4. A cotton plant or a part thereof, or seed therefrom obtained from the seed of claim 3 comprising elite event EE-GH5.
5. A cotton plant, seed, cells, or tissues thereof, each comprising elite event EE-GH5 in its genome, obtained by propagation of and/or breeding with a cotton plant grown from the seed deposited at the ATCC under deposit number PTA-8171.
6. A cotton seed comprising elite event EE-GH5, reference seed comprising said event having been deposited at the ATCC under deposit number PTA-8171.
7. A transgenic cotton plant, cell, or tissue, comprising elite event EE-GH5, produced from the seed of claim 6 .
8. A method for producing a cotton plant or seed comprising elite event EE-GH5 comprising crossing a plant according to claim 1 with another cotton plant, and planting the seed obtained from said cross.
9. A method for determining the zygosity status of a plant, plant material or seed comprising elite event EE-GH5 comprising amplifying DNA fragments of between 100 and 500 bp from a nucleic acid present in said plant, plant material or seed using a polymerase chain reaction with at least three primers, two of said primers specifically recognizing the 5′ or 3′ flanking region of EE-GH5, said 5′ flanking region comprising the nucleotide sequence of SEQ ID No 1 from nucleotide 1 to nucleotide 98 or the nucleotide sequence of SEQ ID No 16 from nucleotide 1 to nucleotide 563, or the 3′ flanking region of EE-GH5, said 3′ flanking region comprising the nucleotide sequence of the complement of SEQ ID No 2 from nucleotide 41 to nucleotide 452 or the pre-insertion DNA comprising the nucleotide sequence of SEQ ID No 15 or the complement thereof, the third of said primers recognizing a sequence within the foreign DNA comprising the nucleotide sequence of the complement of SEQ ID No. 1 from nucleotide 99 to nucleotide 334 or the nucleotide sequence of SEQ ID No 2 from nucleotide 1 to nucleotide 40.
10. The method according to claim 9 , wherein said first primer comprises the nucleotide sequence of SEQ ID No 6, said second primer comprises the nucleotide sequence of SEQ ID No 9 and said third primer comprises the nucleotide sequence of SEQ ID No 3.