Transgenic mouse with a homozygous mutation in the CMAH gene
View Patent ↗This application is in the field of sialic acid chemistry, metabolism, antigenicity, and the production of transgenic non-human mammals with altered sialic acid production. More particularly, this application relates to N-glycolylneuraminic acid (Neu5Gc) being an immunogen in humans, and the production of Neu5Gc-free mammalian products for laboratory and human use.
1. A viable and fertile transgenic mouse that
a) comprises a genome having a homozygous mutation of the cytidine-5′-monophosphate N-acetyl neuraminic acid hydrolase (CMAH) gene,
b) lacks expression of enzymatically active CMAH protein encoded by said CMAH gene, and
c) lacks N-glycolylneuraminic acid (Neu5Gc) in one or more body fluid or tissue.
2. The mouse of claim 1 , wherein said body fluid is selected from the group consisting of serum and milk.
3. The mouse of claim 1 , wherein said tissue comprises muscle.