Nucleic acid sequences encoding transcription factors regulating nicotine alkaloid biosynthesis and their use in modifying plant metabolism
View Patent ↗Plant metabolism and alkaloid levels can be regulated by transcription factors that regulate the nicotinic alkaloid biosynthetic pathway. In one embodiment, the disclosure provides a transcription factor that negatively regulates alkaloid biosynthesis, such as nicotine biosynthesis.
1. An expression vector comprising a nucleic acid molecule comprising a nucleotide sequence wherein the nucleotide sequence is selected from the group consisting of:
(a) the nucleotide sequence set forth in SEQ ID NO: 14 or SEQ ID NO: 15;
(b) a nucleotide sequence that encodes a polypeptide having the amino acid sequence set forth in SEQ ID NO: 16; and
(c) a nucleotide sequence that is at least 90% identical to the nucleotide sequences of (a) or (b), and encodes a transcription factor that negatively regulates nicotine biosynthesis;
wherein the nucleic acid molecule is operably linked to one or more heterologous control sequences suitable for directing expression in a Nicotiana cell.
2. A Nicotiana cell comprising the expression vector of claim 1 .
3. The Nicotiana cell of claim 2 , wherein the Nicotiana cell comprises the transcription factor that negatively regulates nicotine biosynthesis.
4. The Nicotiana cell of claim 3 , wherein the level of the transcription factor in the Nicotiana cell is higher than in a Nicotiana cell that does not contain the expression vector.
5. A nucleic acid molecule comprising a nucleotide sequence wherein the nucleotide sequence is selected from the group consisting of:
(a) the nucleotide sequence set forth in SEQ ID NO: 14 or SEQ ID NO: 15;
(b) a nucleotide sequence that encodes a polypeptide having the amino acid sequence set forth in SEQ ID NO: 16; and
(c) a nucleotide sequence that is at least 90% identical to the nucleotide sequences of (a) or (b), and encodes a transcription factor that negatively regulates nicotine biosynthesis;
wherein the nucleic acid molecule is operably linked to one or more heterologous control sequences.
6. A Nicotiana cell comprising the nucleic acid molecule of claim 5 .
7. The Nicotiana cell of claim 6 , wherein the Nicotiana cell comprises the transcription factor that negatively regulates nicotine biosynthesis.
8. The Nicotiana cell of claim 7 , wherein the level of the transcription factor in the Nicotiana cell is higher than in a Nicotiana cell that does not contain the nucleic acid molecule.