An objective of the present invention is to provide a whole-cell bacterial vaccine composition for preventing whooping cough caused by Bordetella parapertussis , comprising whole cells, whole-cell homogenate, or cell lysate of B. parapertussis as an immunogen, and methods for producing them.
1. A method for preventing whooping cough caused by B. parapertussis in a subject, the method comprising steps of:
(a) culturing B. parapertussis cells at 35° C. to 37° C. using filamentous hemagglutinin (FHA) as an index to produce a B. parapertussis culture preparation with a final cell concentration of 10 10 cells/mL;
(b) inactivating the B. parapertussis cells by adding a formalin solution comprising formaldehyde at a final concentration of 0.2% to the B. parapertussis culture preparation; and
(c) administering the inactivated B. parapertussis cells as whole cells, as a whole-cell homogenate thereof, or as a cell lysate thereof to the subject.
2. A method for inducing immunity against B. parapertussis in a subject, the method comprising steps of:
(a) culturing B. parapertussis cells at 35° C. to 37° C. using filamentous hemagglutinin (FHA) as an index to produce a B. parapertussis culture preparation with a final cell concentration of 10 10 cells/mL;
(b) inactivating the B. parapertussis cells by adding a formalin solution comprising formaldehyde at a final concentration of 0.2% to the B. parapertussis culture preparation; and
(c) administering the inactivated B. parapertussis cells as whole cells, as a whole-cell homogenate thereof, or as a cell lysate thereof to the subject.