Non-specific adsorption inhibitor
A non-specific adsorption inhibitor includes a water-soluble polymer obtained by polymerizing a monomer unit that includes a monomer (A) that forms a polymer that has a lower critical solution temperature (LCST) of 80° C. or less in an aqueous solution under normal pressure when subjected to homopolymerization, and a monomer (B) that forms a polymer that does not have the LCST in an aqueous solution under normal pressure when subjected to homopolymerization.
1. A method of inhibiting non-specific adsorption, the method comprising:
applying a non-specific adsorption inhibitor comprising a water-soluble polymer to a solid phase surface
or adding a non-specific adsorption inhibitor comprising a water-soluble polymer to a diluent of a diagnostic agent, a reaction solvent of a diagnostic agent, or a preservative of a diagnostic agent,
wherein the water-soluble polymer is obtained by polymerizing a monomer unit that comprises
30 to 99 wt % of a monomer (A) that forms a polymer that has a lower critical solution temperature (LCST) of 80° C. or less in an aqueous solution under normal pressure,
1 to 70 wt % of a monomer (B) that forms a polymer that does not have the LCST in an aqueous solution under normal pressure, and
0 to 49 wt % of a monomer (C) that comprises at least one of a carboxyl group-comprising anionic monomer, a sulfone group-comprising anionic monomer, an amino group-comprising cationic monomer, and a hydrophobic monomer,
wherein the monomer (A) comprises at least one of N,N-diethylacrylamide, N-isopropylacrylamide, N-n-propylacrylamide, acryloylmorpholine, dimethylaminoethyl methacrylate, and vinyl methyl ether, and the monomer (B) is at least one selected from the group consisting of acrylamide, N,N-dimethylacrylamide, N-hydroxyethylacrylamide, hydroxyethyl acrylate, polyethylene glycol mono(meth)acrylate, polyethylene glycol monomethyl ether(meth)acrylate, glycerol(meth)acrylate, vinyl acetate, glycidyl(meth)acrylate, allyl glycidyl ether, N-vinylacetamide, N-vinylpyrrolidone, and allyl alcohol.
2. The method of claim 1 , comprising applying a non-specific adsorption inhibitor comprising a water-soluble polymer to a solid phase surface, wherein the surface is a part of an instrument or a container.
3. The method of claim 1 , wherein the monomer (B) comprises acrylamide.
4. The method of claim 1 , wherein the monomer (B) comprises N,N-dimethylacrylamide.
5. The method of claim 1 , wherein the monomer unit comprises monomer (C).
6. The method of claim 5 , wherein the monomer (C) comprises at least one of styrenesulfonic acid, isoprenesulfonic acid, and 2-acrylamide-2-methylpropanesulfonic acid.
7. The method of claim 1 , wherein the water soluble polymer has a number average molecular weight in a range of from 1000 to 1,000,000.
8. The method of claim 1 , wherein the water soluble polymer has a number average molecular weight of from 6800 to 8000, and a weight average molecular weight of from 13,000 to 16,000.
9. The method of claim 1 , wherein the monomer (A) comprises N,N-diethylacrylamide and the monomer (B) comprises N,N-dimethylacrylamide.
10. The method of claim 1 , comprising applying said non-specific adsorption inhibitor comprising said water-soluble polymer to a solid phase surface.
11. The method of claim 1 , comprising adding said non-specific adsorption inhibitor comprising said water-soluble polymer to a diluent of a diagnostic agent, a reaction solvent of a diagnostic agent, or a preservative of a diagnostic agent.
12. The method of claim 1 , wherein the water soluble polymer has a molecular weight distribution of 1.5 to 3.
13. A method of inhibiting non-specific adsorption, the method comprising:
applying a non-specific adsorption inhibitor comprising a water-soluble polymer to a solid phase surface
or adding a non-specific adsorption inhibitor comprising a water-soluble polymer to a diluent of a diagnostic agent, a reaction solvent of a diagnostic agent, or a preservative of a diagnostic agent,
wherein the water-soluble polymer is obtained by polymerizing a monomer unit that comprises
a monomer (A) that forms a polymer that has a lower critical solution temperature (LCST) of 80° C. or less in an aqueous solution under normal pressure, and
a monomer (B) that forms a polymer that does not have the LCST in an aqueous solution under normal pressure,
wherein the monomer (A) comprises N,N-diethylacrylamide.
14. The method of claim 13 , wherein the monomer (B) comprises acrylamide.
15. The method of claim 13 , wherein the monomer (B) comprises N,N-dimethylacrylamide.
16. A method of inhibiting non-specific adsorption, the method comprising:
applying a non-specific adsorption inhibitor comprising a water-soluble polymer to a solid phase surface
or adding a non-specific adsorption inhibitor comprising a water-soluble polymer to a diluent of a diagnostic agent, a reaction solvent of a diagnostic agent, or a preservative of a diagnostic agent,
wherein the water-soluble polymer is obtained by polymerizing a monomer unit that comprises
a monomer (A) that forms a polymer that has a lower critical solution temperature (LCST) of 80° C. or less in an aqueous solution under normal pressure, and
a monomer (B) that forms a polymer that does not have the LCST in an aqueous solution under normal pressure,
wherein the water soluble polymer has a molecular weight distribution of 1.5 to 3.
17. The method of claim 16 , wherein the monomer (A) comprises at least one of N,N-diethylacrylamide, N-isopropylacrylamide, N-n-propylacrylamide, acryloylmorpholine, dimethylaminoethyl methacrylate, and vinyl methyl ether and wherein the monomer (B) is at least one selected from the group consisting of acrylamide, N,N-dimethylacrylamide, N-hydroxyethylacrylamide, hydroxyethyl acrylate, polyethylene glycol mono(meth)acrylate, polyethylene glycol monomethyl ether(meth)acrylate, glycerol(meth)acrylate, vinyl acetate, glycidyl(meth)acrylate, allyl glycidyl ether, N-vinylacetamide, N-vinylpyrrolidone, and allyl alcohol.
18. The method of claim 16 , wherein the monomer (A) comprises N,N-diethylacrylamide and the monomer (B) comprises N,N-dimethylacrylamide.
19. The method of claim 16 , comprising applying said non-specific adsorption inhibitor comprising said water-soluble polymer to a solid phase surface.
20. The method of claim 16 , comprising adding said non-specific adsorption inhibitor comprising said water-soluble polymer to a diluent of a diagnostic agent, a reaction solvent of a diagnostic agent, or a preservative of a diagnostic agent.