Polymer two phase system and use thereof
View Patent ↗The present invention relates to a liquid mixture comprising a first polymer, which is a poly(acid), a second polymer, which is a poly(ether), and at least one salt, wherein the molecular weight of the poly(acid) is in the range of 1000-100,000 Da. The second polymer is selected to be capable of forming immiscible aqueous phases in the presence of the poly(acid) and salt. The poly(acid) may be selected from the group consisting of poly(acrylic acid) and poly(methacrylic acid), and the second synthetic polymer may comprise ethylene oxide. The invention may be used for separation of biomolecules, cells or particles.
1. A method of isolating at least one biomolecule or particle from a liquid, which method comprises
(a) combining a first polymer, which is a poly(acid), with a second polymer, which is a hydrophilic poly(ether), and at least one salt, wherein the molecular weight of the poly(acid) is in the range of 1000-100,000 Da;
(b) combining a liquid comprising at least one biomolecule or at least one particle with the system obtained from (a) to form a liquid mixture;
(c) gentle mixing of the liquid mixture obtained from (b) until at least two phases are formed, wherein the liquid mixture comprises from about 4 to 6% of each polymer; and, optionally,
(d) recovering the desired biomolecule or particle from one of the phases.
2. The method of claim 1 , wherein the molecular weight of the poly(acid) ranges from about 1000 to 20,000 Da.
3. The method of claim 1 , wherein the poly(ether) comprises ethylene oxide.
4. The method of claim 1 , wherein in step (a), the polymer(s) has provided localized on a matrix via preferential wetting.
5. The method of claim 1 , wherein
said particle is a single particle or aggregates of particles and wherein said particle or the aggregates are greater than 5 nm and less than 200 microns in diameter.
6. The method of claim 5 , wherein the particle and/or the aggregates are covalently modified with polymers.