Method of switching from immersion objective lens to dry objective lens
View Patent ↗A microscope apparatus capable of removing liquid from an observation field of view of a dry objective lens, when an immersion objective lens is switched to the dry objective lens, is provided. The microscope apparatus includes a specimen XY stage on which a specimen is placed, a dry objective lens and an immersion objective lens that collect light from the specimen, a movable revolver that selectively disposes one of these objective lenses at a position facing the specimen, and a control unit that controls the specimen XY stage and movable revolver such that the relative positions in the XY direction are changed until the immersion objective lens is disposed at a non-observation region of the dry objective lens, prior to switching of these objective lenses.
1. A control method of an objective lens of a microscope apparatus, the method comprising:
performing an observation of a specimen on a stage with one of an immersion objective lens and a dry objective lens;
selecting to switch from the immersion objective lens to the dry objective lens;
changing a relative position of the immersion objective lens in a direction perpendicular to an optical axis so as to dispose the immersion objective lens in a non-observation region of the dry objective lens while keeping liquid between the immersion objective lens and the specimen due to surface tension, before switching the immersion objective lens to the dry objective lens;
switching from the immersion objective lens to the dry objective lens;
changing the relative position in the direction perpendicular to the optical axis back to an original position; and
performing an observation of the specimen with the dry objective lens.
2. The control method according to claim 1 , wherein the relative position in the direction perpendicular to the optical axis is changed after changing the relative position in the direction along the optical axis within a distance such that liquid between the immersion objective lens and the stage does not separate.