Method and apparatus for forming an homogeneous mixture of chromatography media in a vessel
View Patent ↗The present invention relates to methods and apparatus for forming an homogeneous mixture of chromatography media in a vessel. The invention also relates to methods and apparatus for transferring the homogeneous media from the vessel into a second vessel in preparation for packing a chromatography column. The invention can also be used directly to pack the column with homogeneous media.
1. An apparatus for forming a homogeneous mixture of chromatographic media in a vessel containing said media, the apparatus comprising:
a tubular member ( 10 ), said member comprising a first tube ( 20 ) and a second tube ( 30 ) wherein,
(a) said first tube ( 20 ) comprises an elongate body ( 21 ) having a first orifice ( 22 ) for entry of liquid or slurry therein and a second orifice ( 24 ) for exit of liquid/supernatant or slurry therefrom; and
(b) said second tube ( 30 ) comprising an elongate body ( 31 ) having a first port ( 32 ) for entry of liquid or slurry therein and a second port ( 34 ) for exit of liquid or slurry therefrom, and wherein said tubular member is configured to simultaneously aspirate slurry from the vessel and spray the slurry back into the vessel to form said homogenous mixture of chromatographic media and wherein the apparatus is movable and the tubular member is a hand held rod.
2. The apparatus of claim 1 , wherein the first tube ( 20 ) is concentric with said second tube ( 30 ).
3. The apparatus of claim 1 , wherein the first tube additionally comprises a third orifice for insertion of said second tube therein.
4. The apparatus of claim 1 , wherein the second tube is the inner tube and the first tube is the outer tube of the tubular member.
5. The apparatus of claim 1 , additionally comprising a nozzle attached to said second port for exit of liquid or slurry therefrom.
6. The apparatus of claim 5 , wherein said nozzle is disposed at an angle which is greater than or equal to 90 degrees to the elongate body of the second tube to prevent liquid or slurry being aspirated into the first orifice of the first tube.
7. The apparatus of claim 6 , wherein the nozzle additionally comprises a flow diverter to produce said radial spray.
8. The apparatus of claim 1 , wherein the first tube and the second tube are attached to each other by a releasable fixture to form the tubular member.
9. The apparatus of claim 8 , wherein said releasable fixture is a locking ring.
10. The apparatus of claim 1 , wherein said first tube and/or said second tube are composed of stainless steel.
11. The apparatus of claim 1 , additionally comprising a removable filter unit ( 415 ) positionable over the first orifice ( 22 ) and second port ( 34 ).