DIAGNOSIS OF FETAL ABNORMALITIES BY COMPARATIVE GENOMIC HYBRIDIZATION ANALYSIS
The present invention provides systems, apparatuses, and methods to detect the presence of fetal cells when mixed with a population of maternal cells in a sample and to test fetal abnormalities, e.g. aneuploidy. The present invention involves performing comparative genomic hybridization (CGH) analysis when fetal cells are present in a mixed population of cells. The present invention involves detecting the presence of fetal cells in a mixed maternal sample by detecting the presence of non-maternal alleles in said sample. Furthermore, the present invention also involves correlating the presence of fetal cells in a mixed sample with CGH analysis results to detect a fetal abnormality or declare a test non-informative.
1 . A method for determining a fetal abnormality comprising:
a) enriching one or more fetal cells from a maternal blood sample, by
b) applying said sample to a device comprising an array of obstacles on a substrate,
c) isolating fetal genomic DNA from said fetal cells
d) labeling the resulting fetal DNA fragments with a first label,
e) isolating genomic DNA from a reference sample that is substantially free of fetal cells,
f) labeling the resulting maternal DNA fragments with a second label,
g) hybridizing the fetal and maternal DNA fragments to one or more probes,
h) determining said fetal abnormality based on the hybridization levels of the fetal and maternal DNA fragments.
2 - 79 . (canceled)