VACCINE COMPOSITIONS AND METHODS OF USE
Described are a method and a composition for delivery of a protein to an antigen presenting cell. The composition is composed of a polypeptide component, a buffering component and a particle to be phagocytized. In one embodiment, the antigen presenting cell is aa macrophage or a dendritic cell and the particle to be phagocytized is from a natural source, such as from a microbial source. The composition itself, or cells pretreated with the composition, are useful for strategies in vaccine development.
1 . An antigenic composition comprising (i) a polypeptide component, (ii) a buffering component, and (iii) a particle that can be phagocytosed, wherein the polypeptide component or a fragment thereof is ultimately presented on a class I MHC molecule.
2 . The composition of claim 1 , wherein the particle that can be phagocytosed is a biodegradable particle.
3 . The composition of claim 2 , wherein the particle that can be phagocytosed is a zymosan particle.
4 . The composition of claim 1 , wherein the buffering component is RCONHNH 2 , oligohistidine, or polyethyleneimine.
5 . A vaccine composition comprising (i) a polypeptide component, (ii) a buffering component, and (iii) a particle that can be phagocytosed, wherein the polypeptide component or a fragment thereof is delivered in an amount sufficient to provoke a CD8 T cell response, and the polypeptide component is ultimately presented on a class I MHC molecule.
6 . The vaccine of claim 5 , wherein the particle that can be phagocytosed is a biodegradable particle.
7 . The vaccine of claim 6 , wherein the particle that can be phagocytosed is a zymosan particle.
8 . The vaccine of claim 5 , wherein the buffering component has a buffering capacity in the range of about pH 6 to about pH 8.
9 . The vaccine of claim 5 , wherein the buffering component is RCONHNH 2 , oligohistidine, or polyethyleneimine.
10 . A method for efficient delivery of a polypeptide component to an antigen presenting cell comprising administering a composition comprising (i) a polypeptide component, (ii) a buffering component, and (iii) a particle that can be phagocytosed, wherein the polypeptide component, following administration, enters the cytosol from an endocytotic vesicle, and the polypeptide or a fragment thereof is presented on a MHC class I molecule.
11 . The method of claim 10 , wherein the particle that can be phagocytosed is a biodegradable particle.
12 . The method of claim 11 , wherein the particle that can be phagocytosed is a zymosan particle.
13 . The method of claim 10 , wherein the buffering component is RCONHNH 2 , oligohistidine, or polyethyleneimine.
14 . A composition for exogenous antigen presentation on class I MHC molecules comprising (i) a polypeptide component, (ii) a buffering component, and (iii) a particle that can be phagocytosed.
15 . The composition of claim 14 , wherein the particle that can be phagocytosed is a biodegradable particle.
16 . The composition of claim 15 , wherein the particle that can be phagocytosed is a zymosan particle.
17 . The composition of claim 14 , wherein the buffering component is RCONHNH 2 , oligohistidine, or polyethyleneimine.