Genetic loci associated with cell wall digestibility in maize
The invention relates to methods and compositions for identifying maize plants that have increased cell wall digestibility. The methods use molecular markers to identify and select plants with increased cell wall digestibility or to identify and counter-select plants with decreased cell wall digestibility. Maize plants generated by the methods of the invention are also a feature of the invention.
1. A method of identifying a maize plant that displays increased cell wall digestibility, the method comprising detecting in the germplasm of the maize plant the presence of at least one allele of a marker locus wherein:
a. the marker locus is located within a chromosomal interval comprising and flanked by PHM8098 and PHM9167; and
b. the at least one allele is associated with increased cell wall digestibility.
2. The method of claim 1 , wherein the marker locus is SCW — 04 — 5b.
3. The method of claim 1 , wherein the maize plant belongs to the Stiff Stalk heterotic group.
4. A method of identifying a maize plant that displays increased cell wall digestibility, the method comprising detecting in the germplasm of the maize plant a halotype comprising alleles at one or more marker loci, wherein:
a. the one or more marker loci are located within a chromosomal interval comprising and flanked by PHM8098 and PHM9167; and
b. the halotype is associated with increased cell wall digestibility.
5. The method of claim 4 wherein the one or more marker loci are located within a chromosomal interval comprising and flanked by PHM8098 and PHM9167.
6. The method of claim 4 , wherein the maize plant belongs to the Stiff Stalk heterotic group.
7. A method of marker assisted selection comprising:
a. obtaining a first maize plant having at least one allele of a marker locus, wherein the marker locus is located within a chromosomal interval comprising and flanked by PHM8098 and PHM9167 and the allele of said marker locus is associated with increased cell wall digestibility,
b. crossing said first maize plant to a second maize plant;
c. evaluating the progeny for at least said allele; and
d. selecting progeny plants that possess at least said allele.
8. The method of claim 1 , further comprising after the detecting step the step of introgressing the at least one allele into a maize plant.
9. The method of claim 4 , further comprising after the detecting step the step of introgressing the haplotype into a maize plant.