Glutamate-enhanced cell-penetrating peptides and methods of using same
View Patent ↗Disclosed herein are novel glutamate-enhanced cell-penetrating peptides (CPPs) for use as delivery vehicles to mediate intracellular uptake of therapeutic payloads and methods of using the same.
1. A cell-penetrating peptide comprising SEQ ID. NO 3.
2. The cell-penetrating peptide of claim 1 , further comprising a peptide linker.
3. The cell-penetrating peptide of claim 2 , wherein the linker comprises alanine, glycine, or a combination thereof.
4. The cell-penetrating peptide of claim 1 , further comprising a nuclear localization signal comprising SEQ. ID NO. 4.
5. The cell-penetrating peptide of claim 4 , wherein the cell-penetrating peptide is bound to the N-terminus of the nuclear localization signal.
6. The cell-penetrating peptide of claim 1 , further comprising a therapeutic payload.
7. The cell-penetrating peptide of claim 1 , further comprising a therapeutic payload wherein the therapeutic payload is bound to the C-terminus of the nuclear localization signal NLS peptide.
8. The cell-penetrating peptide of claim 6 , wherein the therapeutic payload comprises a nucleic acid, a liposome, a diagnostic molecule, or a combination thereof.
9. The cell-penetrating peptide of claim 6 , further comprising a FITC label.
10. The cell-penetrating peptide of claim 6 , wherein the therapeutic payload inhibits the growth of a LN-CaP cell.
11. The cell-penetrating peptide of claim 6 , wherein the therapeutic payload comprises a STAT3-inhibiting oligonucleotide.
12. The cell-penetrating peptide of claim 6 , wherein the therapeutic payload comprises SEQ ID NO: 9.
13. A composition comprising a cell-penetrating peptide of claim 1 and a STAT3-inhibiting therapeutic payload.