Recombinant lubricin molecules and uses thereof
View Patent ↗Recombinant lubricin molecules and uses thereof. Novel recombinant lubricin molecules and their uses as lubricants, anti-adhesive agents and/or intra-articular supplements for, e.g., synovial joints, meniscus, tendon, peritoneum, pericardium and pleura, are provided.
1. An isolated protein comprising the polypeptide of SEQ ID NO: 11.
2. The protein of claim 1 , wherein the protein is O-linked to β-(1-3)-Gal-GalNac.
3. A composition comprising a therapeutically effective amount of the protein of claim 2 in a pharmaceutically acceptable carrier.
4. The composition of claim 3 , additionally comprising hyaluronan or hylan.
5. The composition of claim 3 , wherein the composition is formulated for injection.
6. The composition of claim 3 , wherein the composition is encapsulated.
7. The composition of claim 6 , wherein the composition is encapsulated in an implantable drug delivery matrix.
8. The composition of claim 3 , wherein the composition further comprises a local anesthetic.
9. The composition of claim 3 , wherein the composition further comprises a cytokine.
10. The composition of claim 3 , wherein the composition further comprises a lymphokine.
11. The composition of claim 3 , wherein the composition further comprises a thrombolytic factor.
12. The composition of claim 3 , wherein the composition further comprises an antithrombolytic factor.
13. The composition of claim 3 , wherein the composition further comprises an anti-inflammatory agent.
14. The composition of claim 3 , wherein the composition further comprises an antibiotic.
15. The composition of claim 3 , wherein the composition further comprises a growth factor.
16. A method of enhancing the lubrication of a tissue of a subject in need thereof, comprising administering to the tissue of the subject the composition of claim 3 , wherein the tissue is lubricated.
17. The method of claim 16 , wherein the tissue is selected from the group consisting of cartilage, synovium, meniscus, tendon, lung pleura, pericardium, and peritoneum.
18. A method of countering undesired adhesion between tissues in a subject in need thereof, comprising administering to at least one tissue of the subject the composition of claim 3 , wherein the undesired adhesion between the tissues is countered.
19. A method for lubricating the surface of a tissue of a subject in need thereof, comprising administering to the tissue of the subject the composition of claim 3 , wherein the surface of the tissue is lubricated.
20. The method of claim 19 , wherein the tissue is selected from the group consisting of cartilage, synovium, meniscus, tendon, lung pleura, pericardium, and peritoneum.