Recombinant lubricin molecules and uses thereof
View Patent ↗Recombinant lubricin molecules and uses thereof. Novel recombinant lubricin molecules and their uses as lubricants, anti-adhesive agents and/or intra-articular supplements for, e.g., synovial joints, meniscus, tendon, peritoneum, pericardium and pleura, are provided.
1. A method of enhancing the lubrication of a tissue of a subject in need thereof, comprising administering to the tissue of the subject a composition comprising a therapeutically effective amount of an isolated protein comprising the polypeptide of SEQ ID NO:7, wherein the lubrication of the tissue is enhanced.
2. The method of claim 1 , wherein the enhanced lubrication is of a surface of a tissue.
3. The method of claim 1 , wherein the protein is O-linked to β-(1-3)-Gal-GalNac, keratin sulfate, or chondroitin sulfate.
4. The method of claim 1 , wherein the composition further comprises a pharmaceutically acceptable carrier.
5. The method of claim 1 , wherein the composition additionally comprises hyaluronan or hylan.
6. The method of claim 1 , wherein the composition is formulated for injection.
7. The method of claim 1 , wherein the composition is encapsulated.
8. The method of claim 7 , wherein the composition is encapsulated in an implantable drug delivery matrix.
9. The method of claim 1 , wherein the composition further comprises a local anesthetic.
10. The method of claim 1 , wherein the composition further comprises an anti-inflammatory agent.
11. The method of claim 1 , wherein the composition further comprises an antibiotic.
12. The method of claim 1 , wherein the composition further comprises a growth factor.
13. The method of claim 1 , wherein the composition further comprises a cytokine.
14. The method of claim 1 , wherein the composition further comprises a lymphokine.
15. The method of claim 1 , wherein the composition further comprises a thrombolytic factor.
16. The method of claim 1 , wherein the composition further comprises an antithrombolytic factor.
17. The method of claim 1 , wherein the tissue is selected from the group consisting of cartilage, synovium, meniscus, tendon, lung pleura, pericardium, and peritoneum.
18. A method of reducing undesired adhesion between tissues in a subject in need thereof, comprising administering to at least one tissue of the subject a composition comprising a therapeutically effective amount of an isolated protein comprising the polypeptide of SEQ ID NO:7, wherein the undesired adhesion between the tissues is reduced.
19. The method of claim 18 , wherein the protein is O-linked to β-(1-3)-Gal-GalNac, keratin sulfate, or chondroitin sulfate.
20. The method of claim 18 , wherein the composition further comprises a pharmaceutically acceptable carrier.
21. The method of claim 18 , wherein the composition additionally comprises hyaluronan or hylan.
22. The method of claim 18 , wherein the composition is formulated for injection.
23. The method of claim 18 , wherein the composition is encapsulated.
24. The method of claim 23 , wherein the composition is encapsulated in an implantable drug delivery matrix.
25. The method of claim 18 , wherein the composition further comprises a local anesthetic.
26. The method of claim 18 , wherein the composition further comprises an anti-inflammatory agent.
27. The method of claim 18 , wherein the composition further comprises an antibiotic.
28. The method of claim 18 , wherein the composition further comprises a growth factor.
29. The method of claim 18 , wherein the composition further comprises a cytokine.
30. The method of claim 18 , wherein the composition further comprises a lymphokine.
31. The method of claim 18 , wherein the composition further comprises a thrombolytic factor.
32. The method of claim 18 , wherein the composition further comprises an antithrombolytic factor.
33. The method of claim 18 , wherein the tissue is selected from the group consisting of cartilage, synovium, meniscus, tendon, lung pleura, pericardium, and peritoneum.
34. A method for lubricating the surface of a tissue of a subject in need thereof, comprising administering to the tissue of the subject a composition comprising a therapeutically effective amount of an isolated protein comprising the polypeptide of SEQ ID NO: 7, wherein the surface of the tissue is lubricated.