High throughput screening platform for high ethanol production
View Patent ↗Provided herein are high throughput methods for converting starch-containing plant material to ethanol, wherein the conversion process is performed at a small scale. This high-throughput screening platform permits the evaluation of the ethanol obtained from starch-containing plant material in a rapid and efficient manner. The methods include obtaining a plurality of samples of starch-containing plant material and drying the samples to achieve a desired moisture level. The dried samples are liquefied under conditions sufficient to hydrolyze the starch-containing plant material to soluble dextrinized substrates. The liquefied samples are fermented in small volume headspace vials for a period of less than about 96 hours, and fermentation is terminated by pasteurization. Ethanol production is evaluated using headspace gas chromatography.
1. A high throughput method for converting starch-containing plant material to one or more fermentation products comprising:
providing a plurality of samples comprising starch-containing plant material;
drying said samples to achieve a specific moisture level;
liquefying each sample by heating the sample in a convected oven to obtain a liquefact,
fermenting the liquefact of each sample in a small volume container under conditions sufficient for obtaining one or more fermentation products;
pasteurizing said samples under conditions sufficient to terminate fermentation;
wherein one or more fermentation products is produced.
2. The method of claim 1 , wherein said samples are dried to a terminal moisture level.
3. The method of claim 1 , wherein said fermentation product is ethanol.
4. The method of claim 1 , wherein the liquefying step comprises addition of one or more starch-degrading enzymes.
5. The method of claim 1 , wherein the starch-containing plant material is processed prior to the step of drying said samples.
6. The method of claim 1 , wherein said method is modeled for length of time or temperature, or both, of liquefaction.
7. The method of claim 1 , wherein said method is modeled for the source of starch-containing plant material.
8. The method of claim 1 , wherein said method is modeled for the pH of the liquefying step.
9. The method of claim 1 , wherein said method further comprises a saccharification step.
10. The method of claim 1 , further comprising a step of weighing an amount of each of the dried samples for the liquefying step, wherein said weighing is performed in a climate-controlled glove box set at a relative humidity sufficient to maintain the specific moisture level of said samples.
11. The method of claim 3 , wherein the ethanol is evaluated using headspace gas chromatography.
12. The method of claim 4 , wherein the one or more starch-degrading enzymes comprises alpha-amylase.
13. The method of claim 4 , wherein said method is modeled for the source of starch-degrading enzyme, the type of starch-degrading enzyme, the specific combination of starch-degrading enzymes, or any combination thereof.
14. The method of claim 5 , wherein the starch-containing plant material is processed by milling said plant material.
15. The method of claim 9 , wherein said saccharification step is a simultaneous liquefaction and saccharification step.
16. The method of claim 9 , wherein said saccharification step is a simultaneous saccharification and fermentation step.